PUS10 Protein (Myc-DYKDDDDK Tag)
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- Target See all PUS10 Proteins
- PUS10 (Pseudouridylate Synthase 10 (PUS10))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PUS10 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human PUS10 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PUS10 Protein
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Pseudouridylate Synthase 10 (PUS10) (AA 1-529) protein (Strep Tag)
Crystallography grade PUS10 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Pseudouridylate Synthase 10 (PUS10) (AA 1-527) protein (Strep Tag)Crystallography grade PUS10 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PUS10 (Pseudouridylate Synthase 10 (PUS10))
- Alternative Name
- Pus10 (PUS10 Products)
- Synonyms
- CCDC139 Protein, DOBI Protein, 2810013G11Rik Protein, 4933435A13Rik Protein, AU014648 Protein, C77560 Protein, Ccdc139 Protein, RGD1306402 Protein, pseudouridylate synthase 10 Protein, PUS10 Protein, Pus10 Protein
- Background
- Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM, May 2009]
- Molecular Weight
- 60.1 kDa
- NCBI Accession
- NP_653310
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