RFX5 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2730796

Product Details

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This RFX5 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human RFX5 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RFX5 (Regulatory Factor X 5 (RFX5))

Alternative Name: Rfx5 (RFX5 Products)

Synonyms: regulatory factor X5 Protein, regulatory factor X, 5 (influences HLA class II expression) Protein, RFX5 Protein, Rfx5 Protein

Background: A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.

Molecular Weight: 65.1 kDa

NCBI Accession: NP_001020774