Ring Finger Protein 25 (RNF25) protein (Myc-DYKDDDDK Tag)

Details for Product No. ABIN2731045, Supplier: Log in to see
Protein Name
  • AO7
  • 0610009H16Rik
  • ring finger protein 25
  • RNF25
  • Rnf25
Origin
Human
3
1
1
Source
HEK-293 Cells
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This RNF25 protein is labelled with Myc-DYKDDDDK Tag.
Application
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Options
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Specificity Optimal preservation of protein structure, post-translational modifications and functions.
Characteristics
  • Recombinant human RNF25 protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Purity > 80 % as determined by SDS-PAGE and Coomassie blue staining
Background The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.
Molecular Weight 51 kDa
NCBI Accession NP_071898
Research Area Proteolysis / Ubiquitin, Ion Channels
Application Notes Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Comment

The tag is located at the C-terminal.

Restrictions For Research Use only
Concentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Storage -80 °C
Storage Comment Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Supplier Images
Western Blotting (WB) image for Ring Finger Protein 25 (RNF25) protein (Myc-DYKDDDDK Tag) (ABIN2731045) Validation with Western Blot