RPE65 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2731119

Product Details

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This RPE65 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human RPE65 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: RPE65 (Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65))

Alternative Name: Rpe65 (RPE65 Products)

Synonyms: LCA2 Protein, RP20 Protein, mRPE65 Protein, rd12 Protein, sRPE65 Protein, 65kDa Protein, A930029L06Rik Protein, Mord1 Protein, DRPE65 Protein, RPE65 Protein, anon-WO0148162.1 Protein, anon-WO0148163.1 Protein, beta-diox Protein, DmelCG9347 Protein, CG9347 Protein, MGC52887 Protein, MGC154296 Protein, MGC85437 Protein, rpe65 Protein, RPE56c Protein, si:ch211-198n5.12 Protein, 13cIMH Protein, RPE56b Protein, rpepb Protein, si:ch211-198n5.5 Protein, wu:fc74c09 Protein, zgc:110538 Protein, RPE56a Protein, rpepa Protein, zgc:73213 Protein, RPE65, retinoid isomerohydrolase Protein, retinal pigment epithelium 65 Protein, neither inactivation nor afterpotential B Protein, retinal pigment epithelium-specific protein 65kDa S homeolog Protein, retinal pigment epithelium-specific protein 65kDa L homeolog Protein, RPE65 homolog Protein, retinal pigment epithelium-specific protein 65kDa Protein, retinal pigment epithelium-specific protein 65c Protein, retinal pigment epithelium-specific protein 65b Protein, retinal pigment epithelium-specific protein 65a Protein, RPE65 Protein, Rpe65 Protein, ninaB Protein, rpe65.S Protein, rpe65.L Protein, rpe65 Protein, rpe65c Protein, rpe65b Protein, rpe65a Protein

Background: This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa.

Molecular Weight: 60.8 kDa

NCBI Accession: NP_000320