EPT1 Protein (His tag)

Catalog No. ABIN2731619

Product Details

Target: EPT1 (ethanolaminephosphotransferase 1 (CDP-Ethanolamine-Specific) (EPT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EPT1 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Selenoprotein I (SELI) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 20 mM Tris-HCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: EPT1 (ethanolaminephosphotransferase 1 (CDP-Ethanolamine-Specific) (EPT1))

Alternative Name: Selenoprotein I (Seli) (EPT1 Products)

Synonyms: SELI Protein, SEPI Protein, 4933402G07Rik Protein, AI448296 Protein, AI452230 Protein, C79563 Protein, D5Wsu178e Protein, mKIAA1724 Protein, hEPT1 Protein, RGD1560938 Protein, EPT1 Protein, selenoprotein I Protein, SELENOI Protein, Selenoi Protein

Background: The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 32.6 kDa

NCBI Accession: NP_277040