SEMG1 Protein (Transcript Variant 2) (His tag)

Catalog No. ABIN2731629

Product Details

Target: SEMG1 (Semenogelin I (SEMG1))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 2

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SEMG1 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human Semenogelin I (SEMG1), transcript variant 2 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM Hac-NaAc, 150 mM NaCl, pH 4.5

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SEMG1 (Semenogelin I (SEMG1))

Abstract: SEMG1 Products

Synonyms: SEMG1 Protein, SEMG1a Protein, CT103 Protein, SEMG Protein, SGI Protein, dJ172H20.2 Protein, RATSVPIIA Protein, SVPIIA Protein, SVS2P Protein, Svp1 Protein, Svs2 Protein, Svs2p2 Protein, BB115391 Protein, Semg1 Protein, SvsII Protein, semenogelin II Protein, semenogelin I Protein, semenogelin-2 Protein, seminal vesicle secretory protein 2 Protein, SEMG2 Protein, SEMG1 Protein, LOC100408385 Protein, Semg1 Protein, Svs2 Protein

Background: The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. This preproprotein is proteolytically processed by the prostate-specific antigen (PSA) protease to generate multiple peptide products that exhibit distinct functions. One of these peptides, SgI-29, is an antimicrobial peptide with antibacterial activity. This proteolysis process also breaks down the gel matrix and allows the spermatozoa to move more freely. This gene and another similar semenogelin gene are present in a gene cluster on chromosome 20.

Molecular Weight: 43.8kD

NCBI Accession: NP_937782