SF3B3 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2731901

Product Details

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This SF3B3 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human SF3B3 / SAP130 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

Alternative Name: Sf3b3,sap130 (SF3B3 Products)

Synonyms: GB11574 Protein, rse1 Protein, sap130 Protein, sf3b130 Protein, staf130 Protein, sf3b3 Protein, 1810061H24Rik Protein, 5730409A01Rik Protein, AA409318 Protein, D8Ertd633e Protein, RSE1 Protein, SAP130 Protein, mKIAA0017 Protein, ik:tdsubc_2b2 Protein, wu:fb81f05 Protein, xx:tdsubc_2b2 Protein, zgc:55440 Protein, SF3b130 Protein, STAF130 Protein, splicing factor 3b subunit 3 Protein, splicing factor 3B subunit 3 Protein, splicing factor 3b, subunit 3, 130kDa Protein, splicing factor 3b subunit 3 L homeolog Protein, splicing factor 3b, subunit 3 Protein, SF3B3 Protein, LOC550935 Protein, sf3b3 Protein, Sf3b3 Protein, LOC100178056 Protein, sf3b3.L Protein

Background: This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Molecular Weight: 135.4 kDa

NCBI Accession: NP_036558