SLC25A27 Protein (His tag)

Catalog No. ABIN2732159

Product Details

Target: SLC25A27 (Solute Carrier Family 25, Member 27 (SLC25A27))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SLC25A27 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human SLC25A27 / UCP4 (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SLC25A27 (Solute Carrier Family 25, Member 27 (SLC25A27))

Alternative Name: Slc25a27,ucp4 (SLC25A27 Products)

Synonyms: UCP4 Protein, Ucp4 Protein, 3632410G24Rik Protein, 9430092A03Rik Protein, D530043E16Rik Protein, solute carrier family 25 member 27 Protein, solute carrier family 25, member 27 Protein, SLC25A27 Protein, Slc25a27 Protein

Background: Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. Transcripts of this gene are only detected in brain tissue and are specifically modulated by various environmental conditions. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011].

Molecular Weight: 35.9 kDa

NCBI Accession: NP_004268