SMAD4 Protein (Myc-DYKDDDDK Tag)
(1 reference)-
- Target See all SMAD4 Proteins
- SMAD4 (SMAD Family Member 4 (SMAD4))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SMAD4 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human SMAD4 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product SMAD4 Protein
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SMAD Family Member 4 (SMAD4) (AA 1-552) protein (GST tag)
SMAD4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD Family Member 4 (SMAD4) (AA 13-227) protein (His tag)SMAD4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
SMAD Family Member 4 (SMAD4) (AA 56-165) protein (GST tag)SMAD4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
SMAD Family Member 4 (SMAD4) (AA 1-552) protein (His tag)Crystallography grade SMAD4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
SMAD Family Member 4 (SMAD4) (AA 1-551) protein (His tag)Crystallography grade SMAD4 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "M10, a caspase cleavage product of the hepatocyte growth factor receptor, interacts with Smad2 and demonstrates antifibrotic properties in vitro and in vivo." in: Translational research : the journal of laboratory and clinical medicine, Vol. 170, pp. 99-111, (2016) (PubMed).
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: "M10, a caspase cleavage product of the hepatocyte growth factor receptor, interacts with Smad2 and demonstrates antifibrotic properties in vitro and in vivo." in: Translational research : the journal of laboratory and clinical medicine, Vol. 170, pp. 99-111, (2016) (PubMed).
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- Target
- SMAD4 (SMAD Family Member 4 (SMAD4))
- Alternative Name
- Smad4 (SMAD4 Products)
- Synonyms
- smad4 Protein, DPC4 Protein, JIP Protein, MADH4 Protein, MYHRS Protein, AW743858 Protein, D18Wsu70e Protein, Madh4 Protein, XSmad4alpha Protein, Xsmad4 Protein, dpc4 Protein, jip Protein, madh4 Protein, xsmad4a Protein, SMAD family member 4 Protein, Smad4 protein Protein, SMAD family member 4, gene 1 L homeolog Protein, SMAD4 Protein, smad4 Protein, Smad4 Protein, smad4.1.L Protein
- Background
- This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
- Molecular Weight
- 60.3 kDa
- NCBI Accession
- NP_005350
- Pathways
- Cell Division Cycle, Chromatin Binding, Autophagy
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