SPI1 Protein (His tag)

Catalog No. ABIN2732581

Product Details

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This SPI1 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human SPI1 / PU.1 (full length, N-term HIS tag, transcript variant 2) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: Spi1,pu.1 (SPI1 Products)

Synonyms: OF Protein, PU.1 Protein, SFPI1 Protein, SPI-1 Protein, SPI-A Protein, Dis-1 Protein, Dis1 Protein, Sfpi-1 Protein, Sfpi1 Protein, Spi-1 Protein, Tcfpu1 Protein, Tfpu.1 Protein, Pu.1 Protein, cb1086 Protein, pu.1 Protein, pu1 Protein, sfpi1 Protein, si:by184l24.2 Protein, spi1 Protein, SPI1/PU.1 Protein, spi1l Protein, zgc:152991 Protein, Spi-1 proto-oncogene Protein, spleen focus forming virus (SFFV) proviral integration oncogene Protein, Spi-1 proto-oncogene b Protein, Spi-1 proto-oncogene a Protein, SPI1 Protein, Spi1 Protein, spi1b Protein, spi1a Protein

Background: This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 30.9 kDa

NCBI Accession: NP_003111

Pathways: Stem Cell Maintenance