TDO2 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2733390

Product Details

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This TDO2 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human TDO2 / TDO protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Biological Activity Comment: The specific activity of TDO2 was determined by monitoring Kynurenine formation from the N-formylkynurenine based on the absorbance at 492nm. The N-formylkynurenine was produced from a conversion of tryptophan with TDO2. The reactions were carried out at 37°C for 40min in 100ul of the reaction volume containing 200mM PBS, pH7.5, 1mM ascorbic acid, and 1.25mM L-tryptophan as the substrate with various amounts of TDO2. The reaction was terminated by adding 50ul of 30% (w/v) trichloroacetic acid. The sample was further incubated for 30min at 60°C and centrifuged at 12000 rpm for 15 min. The supernatant was used to mix with an equal volume of Ehrlich's reagent (2% p-dimethylaminobenza-ldehyde in glacial acetic acid) to measure the absorbance at 492 nm after 10min incubation

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Alternative Name: Tdo2,tdo (TDO2 Products)

Synonyms: Tdo2 Protein, tdo2l Protein, fi30d08 Protein, zgc:63488 Protein, wu:fi30d08 Protein, TDO2 Protein, fb62b10 Protein, tdo2 Protein, wu:fb62b10 Protein, zgc:103693 Protein, DDBDRAFT_0190495 Protein, DDBDRAFT_0231363 Protein, DDB_0190495 Protein, DDB_0231363 Protein, TDO Protein, TO Protein, TPH2 Protein, TRPO Protein, AA407491 Protein, chky Protein, Tdo Protein, tryptophan 2,3-dioxygenase b Protein, tryptophan 2,3-dioxygenase Protein, tryptophan 2,3-dioxygenase a Protein, tryptophan 2,3-dioxygenase L homeolog Protein, tdo2b Protein, tdo2 Protein, TDO2 Protein, MADE_02822 Protein, CtCNB1_3657 Protein, tdo2a Protein, CNC04830 Protein, tdo Protein, Mrub_2119 Protein, Deipr_2235 Protein, Acav_1166 Protein, Mesop_4278 Protein, Tdo2 Protein, tdo2.L Protein

Background: This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism.

Molecular Weight: 47.7 kDa

NCBI Accession: NP_005642