TRMT12 Protein (His tag)
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- Target See all TRMT12 Proteins
- TRMT12 (tRNA Methyltransferase 12 Homolog (TRMT12))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TRMT12 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human TRMT12 (full length, N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product TRMT12 Protein
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tRNA Methyltransferase 12 Homolog (TRMT12) (AA 1-448) protein (GST tag)
TRMT12 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
tRNA Methyltransferase 12 Homolog (TRMT12) (AA 1-448) protein (Strep Tag)Crystallography grade TRMT12 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
tRNA Methyltransferase 12 Homolog (TRMT12) (AA 1-446) protein (Strep Tag)Crystallography grade TRMT12 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- TRMT12 (tRNA Methyltransferase 12 Homolog (TRMT12))
- Alternative Name
- Trmt12 (TRMT12 Products)
- Synonyms
- 4632406N01Rik Protein, AI449275 Protein, Tyw2 Protein, TRM12 Protein, TYW2 Protein, RGD1307127 Protein, trmt12 Protein, tRNA methyltranferase 12 Protein, tRNA methyltransferase 12 homolog Protein, tRNA methyltransferase 12 Protein, tRNA methyltransferase 12 homolog (S. cerevisiae) Protein, Trmt12 Protein, TRMT12 Protein, trmt12 Protein
- Background
- Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006).[supplied by OMIM, Mar 2008].
- Molecular Weight
- 50.2 kDa
- NCBI Accession
- NP_060426
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