ube3a Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2734879

Product Details

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 3

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ube3a protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human UBE3A (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: Ube3a (ube3a Products)

Synonyms: ANCR Protein, AS Protein, E6-AP Protein, EPVE6AP Protein, HPVE6A Protein, DDBDRAFT_0188760 Protein, DDBDRAFT_0302447 Protein, DDB_0188760 Protein, DDB_0302447 Protein, ube3a Protein, im:7140733 Protein, zgc:92173 Protein, 4732496B02 Protein, 5830462N02Rik Protein, A130086L21Rik Protein, Hpve6a Protein, mKIAA4216 Protein, As Protein, CG6190 Protein, Dmel\\CG6190 Protein, Dube3a Protein, UBE3A Protein, dUBE3A Protein, das Protein, dube3A Protein, dube3a Protein, MGC69536 Protein, UME3A Protein, ubiquitin protein ligase E3A Protein, ubiquitin-protein ligase E3A Protein, Ubiquitin protein ligase E3A Protein, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) S homeolog Protein, ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman syndrome) Protein, UBE3A Protein, ube3a Protein, LOC100194730 Protein, Ube3a Protein, ube3a.S Protein, Eint_040430 Protein

Background: This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.

Molecular Weight: 99.9 kDa

NCBI Accession: NP_570854

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway