VHLL Protein (His tag)
VHLL
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
1 image
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- Target See all VHLL Proteins
- VHLL (Von Hippel-Lindau Tumor Suppressor-Like (VHLL))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This VHLL protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human VHLL (full length, N-term HIS tag) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product VHLL Protein
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Von Hippel-Lindau Tumor Suppressor-Like (VHLL) (AA 1-139) protein (Strep Tag)
Crystallography grade VHLL Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the N-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- VHLL (Von Hippel-Lindau Tumor Suppressor-Like (VHLL))
- Abstract
- VHLL Products
- Synonyms
- VHLP Protein, VLP Protein, VHL like Protein, VHLL Protein
- Background
- Von Hippel-Lindau (VHL) tumor suppressor protein is a component of an E3 ubiquitin ligase complex that selectively ubiquitinates the alpha subunit of the hypoxia-inducible factor (HIF) transcription factor for proteasome-mediated degradation. Inactivation of VHL causes VHL disease and sporadic kidney cancer. This gene encodes a VHL homolog that lacks one of two key domains necessary for VHL function. This gene may contribute to the regulation of oxygen homeostasis and neovascularization during placenta development. This gene is intronless, and can also be interpreted as a retrotransposed pseudogene of the VHL locus located on chromosome 3. However, the protein is represented in this RefSeq due to evidence in PMID:14757845 that strongly suggests it is translated. The same publication also indicates that this protein binds HIF alpha but fails to recruit the E3 ubiquitin ligase complex, and it therefore functions as a dominant-negative VHL protein and a protector of HIF alpha.
- Molecular Weight
- 15.6 kDa
- NCBI Accession
- NP_001004319
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