Diphthamide Biosynthesis 7 (DPH7) protein (Myc-DYKDDDDK Tag)
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- Target See all Diphthamide Biosynthesis 7 (DPH7) Proteins
- Diphthamide Biosynthesis 7 (DPH7)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- Myc-DYKDDDDK Tag
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human WDR85 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product DPH7 Protein
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Diphthamide Biosynthesis 7 (DPH7) (AA 1-452) protein (GST tag)
DPH7 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Diphthamide Biosynthesis 7 (DPH7) (AA 1-452) protein (Strep Tag)Crystallography grade DPH7 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Diphthamide Biosynthesis 7 (DPH7) (AA 1-477) protein (Strep Tag)Crystallography grade DPH7 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Diphthamide Biosynthesis 7 (DPH7)
- Alternative Name
- Wdr85 (DPH7 Products)
- Synonyms
- C9orf112 Protein, RP11-48C7.3 Protein, RRT2 Protein, WDR85 Protein, diphthamide biosynthesis 7 Protein, DPH7 Protein
- Background
- Diphthamide is a post-translationally modified histidine residue present in elongation factor 2, and is the target of diphtheria toxin. This gene encodes a protein that contains a WD-40 domain, and is thought to be involved in diphthamide biosynthesis. A similar protein in yeast functions as a methylesterase, converting methylated diphthine to diphthine, which can then undergo amidation to produce diphthamide.
- Molecular Weight
- 50.4 kDa
- NCBI Accession
- NP_620133
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