XRCC4 Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2735698

Product Details

Target: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Protein Type: Recombinant

Protein Characteristics: Transcript Variant 3

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This XRCC4 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human XRCC4 (transcript variant 3) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Myers, Maxwell, Wang: "Quantification of XRCC and DNA-PK proteins in cancer cell lines and human tumors by LC-MS/MS." in: Bioanalysis, Vol. 6, Issue 22, pp. 2969-83, (2014) (PubMed).

Target Details

Target: XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4 (XRCC4))

Alternative Name: Xrcc4 (XRCC4 Products)

Synonyms: zgc:73312 Protein, MGC81443 Protein, homolog of human DNA ligase iv-binding protein XRCC4 Protein, 2310057B22Rik Protein, AW413319 Protein, AW545101 Protein, X-ray repair cross complementing 4 Protein, X-ray repair complementing defective repair in Chinese hamster cells 4 Protein, X-ray repair complementing defective repair in Chinese hamster cells 4 L homeolog Protein, DNA ligase IV-binding protein Protein, XRCC4 Protein, xrcc4 Protein, xrcc4.L Protein, Xrcc4 Protein

Background: The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants.

Molecular Weight: 37.9 kDa

NCBI Accession: NP_072044

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response