ZYX Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all ZYX Proteins
- ZYX (Zyxin (ZYX))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ZYX protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Zyxin (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ZYX Protein
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Zyxin (ZYX) (AA 2-168) protein (His-IF2DI Tag)
ZYX Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Zyxin (ZYX) (AA 1-572) protein (GST tag)ZYX Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Zyxin (ZYX) (AA 2-572) protein (His tag)Crystallography grade ZYX Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Zyxin (ZYX) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ZYX Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- ZYX (Zyxin (ZYX))
- Alternative Name
- Zyxin (ZYX Products)
- Synonyms
- BcDNA:LD06023 Protein, CG32018 Protein, DLim-1 Protein, DmLIM-1 Protein, Dmel\\CG32018 Protein, Lim102EF Protein, ZYX102 Protein, Zyx102 Protein, Zyx102EF Protein, lim Protein, zyx102 Protein, zyxin Protein, zgc:171680 Protein, ZYX Protein, zyx Protein, MGC145270 Protein, ESP-2 Protein, HED-2 Protein, 9530098H06Rik Protein, R75157 Protein, Zyxin Protein, zyxin Protein, zyxin S homeolog Protein, zyx-1 Protein, Zyx Protein, zyx Protein, ZYX Protein, EDI_201420 Protein, zyx.S Protein
- Background
- Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
- Molecular Weight
- 61.1 kDa
- NCBI Accession
- NP_001010972
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