NAPRT1 Protein (His tag)
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- Target See all NAPRT1 (NAPRT) Proteins
- NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NAPRT1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Cross-Reactivity
- Human
- Characteristics
- Human NAPRTase is fused at the N-terminus to a His-tag.
- Purity
- >98 % (SDS-PAGE)
- Top Product
- Discover our top product NAPRT Protein
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Nicotinate phosphoribosyltransferase (NAPRT) (AA 1-466) protein (GST tag)
NAPRT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nicotinate phosphoribosyltransferase (NAPRT) (AA 1-538) protein (Strep Tag)Crystallography grade NAPRT Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Nicotinate phosphoribosyltransferase (NAPRT) (AA 1-538) protein (Strep Tag)Crystallography grade NAPRT Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Nicotinate phosphoribosyltransferase (NAPRT) protein (His tag)NAPRT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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~0.27 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinic acid mononucleotide (NaMN) per min.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- In 50 mM HEPES/KOH pH 7.5 containing 300 mM potassium chloride and 10 mM DTT.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
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Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C. - Expiry Date
- 6 months
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- Target
- NAPRT1 (NAPRT) (Nicotinate phosphoribosyltransferase (NAPRT))
- Alternative Name
- NAPRTase (NAPRT Products)
- Synonyms
- PP3856 Protein, 9130210N20Rik Protein, zgc:77870 Protein, nicotinate phosphoribosyltransferase Protein, NAPRT Protein, Naprt Protein, naprt Protein
- Background
- Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosynthesis pathway. Catalytic activity: Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
- Molecular Weight
- ~58.0kDa
- UniProt
- Q6XQN6
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