NMNAT3 Protein (AA 1-252) (His tag)
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- Target See all NMNAT3 Proteins
- NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-252
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NMNAT3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Cross-Reactivity
- Human
- Characteristics
- Human full-length NMNAT3 (aa 1-252) is fused at the N-terminus to a His-tag.
- Purity
- >95 % (SDS-PAGE)
- Top Product
- Discover our top product NMNAT3 Protein
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Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) (AA 1-252) protein (His tag)
NMNAT3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) (AA 1-215) protein (GST tag)NMNAT3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) (AA 116-215) protein (GST tag)NMNAT3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) (AA 1-245) protein (His tag)Crystallography grade NMNAT3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3) (AA 1-215), (Isoform 2) protein (His-SUMO Tag)NMNAT3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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>2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol of NAD+ per min.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- In 50 mM TRIS-HCl, pH 8.0, containing 300 mM sodium chloride, 1 mM DTT and 10 % glycerol.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C.
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- Target
- NMNAT3 (Nicotinamide Nucleotide Adenylyltransferase 3 (NMNAT3))
- Alternative Name
- NMNAT3 (NMNAT3 Products)
- Background
- Mitochondrial NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Can use tiazofurin monophosphate as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD(+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following injury.
- Molecular Weight
- ~27kDa (monomer)
- UniProt
- Q96T66
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