TDO2 Protein (His tag)

Catalog No. ABIN2745861

Product Details

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mosquito

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This TDO2 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Characteristics: Full-length TDO (Anopheles gambiae) is fused at the N-terminus to a His-tag.

Purity: >98 % (SDS-PAGE)

Endotoxin Level: .

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Specific activity towards L-Trp: 87.0μmol/hr per mg. TDO catalyzes the oxidative opening of the L-tryptophan indole moiety to yield N-formylkynurenine (NFK).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. In 50 mM sodium phosphate pH 8.0, containing 0.15M sodium chloride, 0.2M imidazole and 10 % glycerol.

Storage: -20 °C,-80 °C

Storage Comment: Short Term Storage: -20°C
Long Term Storage: -80°C
Stable for at least 6 months after receipt when stored at -80°C.

Expiry Date: 6 months

Target Details

Target: TDO2 (Tryptophan 2,3-Dioxygenase (TDO2))

Alternative Name: TDO (TDO2 Products)

Synonyms: Tdo2 Protein, tdo2l Protein, fi30d08 Protein, zgc:63488 Protein, wu:fi30d08 Protein, TDO2 Protein, fb62b10 Protein, tdo2 Protein, wu:fb62b10 Protein, zgc:103693 Protein, DDBDRAFT_0190495 Protein, DDBDRAFT_0231363 Protein, DDB_0190495 Protein, DDB_0231363 Protein, TDO Protein, TO Protein, TPH2 Protein, TRPO Protein, AA407491 Protein, chky Protein, Tdo Protein, tryptophan 2,3-dioxygenase b Protein, tryptophan 2,3-dioxygenase Protein, tryptophan 2,3-dioxygenase a Protein, tryptophan 2,3-dioxygenase L homeolog Protein, tdo2b Protein, tdo2 Protein, TDO2 Protein, MADE_02822 Protein, CtCNB1_3657 Protein, tdo2a Protein, CNC04830 Protein, tdo Protein, Mrub_2119 Protein, Deipr_2235 Protein, Acav_1166 Protein, Mesop_4278 Protein, Tdo2 Protein, tdo2.L Protein

Background: Tryptophan 2,3-dioxygenase (TDO) is a heme-containing dioxygenase catalyzing the addition of molecular oxygen across the 2,3-double bond of the indole ring of tryptophan to form N-formylkynurenine (NFK). In Anopheles gambiae, TDO is the only enzyme able to catalyze the first and rate-limiting step in L-Trp catabolism through the kynurenine pathway. Tryptophan is an essential amino acid for protein synthesis and also the precursor for production of a number of neurotransmitters, such as serotonin and melatonin, in mosquitoes, the kynurenine pathway is essential for eye pigmentation. Conceivably, the tryptophan-using pathways should be regulated in a coordinated manner in mosquitoes as well as in other species and TDO activation/inactivation processes could play an essential role in these phenomena.

Molecular Weight: ~47.9kDa

UniProt: O77457