SYCE2 Protein (AA 1-218) (His tag)
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- Target See all SYCE2 Proteins
- SYCE2 (Synaptonemal Complex Central Element Protein 2 (SYCE2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-218
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This SYCE2 protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MERQGVDVPH VKCKDQEPQP LGESKEHPRW EENCEEEAGG GPASASCQLT VLEGKSGLYF SSLDSSIDIL QKRAQELIEN INKSRQKDHA LMTNFRNSLK TKVSDLTEKL EERIYQIYND HNKIIQEKLQ EFTQKMAKIS HLETELKQVC HSVETVYKDL CLQPEQSLRL RWGPDHSRGK SPPRPGNSQP PDVFVSSVAE TTSQATASEV QTNRDGEC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SYCE2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SYCE2 (Synaptonemal Complex Central Element Protein 2 (SYCE2))
- Alternative Name
- SYCE2 (SYCE2 Products)
- Synonyms
- srg Protein, zsrg Protein, MGC136320 Protein, zgc:136320 Protein, CESC1 Protein, 1700013H19Rik Protein, AA407907 Protein, Cesc1 Protein, RGD1311867 Protein, synaptonemal complex central element protein 2 Protein, SYCE2 Protein, syce2 Protein, Syce2 Protein
- Background
- Major component of the transverse central element of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Requires SYCP1 in order to be incorporated into the central element. May have a role in the synaptonemal complex assembly, stabilization and recombination (By similarity). {ECO:0000250}.
- Molecular Weight
- 25.6 kDa Including tag.
- UniProt
- Q6PIF2
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