TSPAN33 Protein (AA 1-283) (Strep Tag)

Catalog No. ABIN3074908

Product Details

Target: TSPAN33 (Tetraspanin 33 (TSPAN33))

Protein Type: Recombinant

Protein Characteristics: AA 1-283

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TSPAN33 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MARRPRAPAA SGEEFSFVSP LVKYLLFFFN MLFWVISMVM VAVGVYARLM KHAEAALACL AVDPAILLIV VGVLMFLLTF CGCIGSLREN ICLLQTFSLC LTAVFLLQLA AGILGFVFSD KARGKVSEII NNAIVHYRDD LDLQNLIDFG QKKFSCCGGI SYKDWSQNMY FNCSEDNPSR ERCSVPYSCC LPTPDQAVIN TMCGQGMQAF DYLEASKVIY TNGCIDKLVN WIHSNLFLLG GVALGLAIPQ LVGILLSQIL VNQIKDQIKL QLYNQQHRAD PWY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TSPAN33 (Tetraspanin 33 (TSPAN33))

Alternative Name: TSPAN33 (TSPAN33 Products)

Synonyms: zgc:92266 Protein, PEN Protein, 1300010A20Rik Protein, AI035228 Protein, Pen Protein, RGD1560915 Protein, tetraspanin 33 Protein, tetraspanin 33a Protein, tetraspanin 33 L homeolog Protein, TSPAN33 Protein, tspan33a Protein, tspan33.L Protein, Tspan33 Protein

Background: Tetraspanin-33 (Tspan-33) (Penumbra) (hPen) (Proerythroblast new membrane),FUNCTION: Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates (PubMed:26686862, PubMed:30463011, PubMed:37516108). Plays an important role in normal erythropoiesis (By similarity). It has a role in the differentiation of erythroid progenitors (By similarity). Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity (PubMed:26686862). Mediates docking of ADAM10 to zonula adherens by interacting with ADAM10 and, in a PDZD11-dependent manner, with the zonula adherens protein PLEKHA7 (PubMed:30463011). {ECO:0000250|UniProtKB:Q8R3S2, ECO:0000269|PubMed:26686862, ECO:0000269|PubMed:30463011, ECO:0000269|PubMed:37516108}.

Molecular Weight: 31.5 kDa

UniProt: Q86UF1