VANGL2 Protein (AA 1-108) (His tag)

Catalog No. ABIN3075255

Product Details

Target: VANGL2 (Vang-Like 2 (VANGL2))

Protein Type: Recombinant

Protein Characteristics: AA 1-108

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This VANGL2 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: MDTESQYSGY SYKSGHSRSS RKHRDRRDRH RSKSRDGGRG DKSVTIQAPG EPLLDNESTR GDERDDNWGE TTTVVTGTSE HSISHDDLTR IAKDMEDSVP LDCSRHLG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human VANGL2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: VANGL2 (Vang-Like 2 (VANGL2))

Alternative Name: VANGL2 (VANGL2 Products)

Synonyms: Strabismus Protein, lpp1 Protein, ltap Protein, stb1 Protein, stbm Protein, stbm1 Protein, VANGL2 Protein, LPP1 Protein, LTAP Protein, STB1 Protein, STBM Protein, STBM1 Protein, C530001F03Rik Protein, Lp Protein, Lpp1 Protein, Ltap Protein, Vang1l2 Protein, loop-tail Protein, ska17 Protein, ska Protein, strabismus Protein, tri Protein, vang Protein, vangl2 Protein, vangl2b Protein, vangl2a Protein, VANGL planar cell polarity protein 2 Protein, vang-like 2 (van gogh, Drosophila) Protein, VANGL planar cell polarity protein 2 S homeolog Protein, VANGL planar cell polarity protein 2 L homeolog Protein, vangl2 Protein, VANGL2 Protein, Vangl2 Protein, vangl2.S Protein, vangl2.L Protein

Background: Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Required for cell surface localization of FZD3 and FZD6 in the inner ear (By similarity). {ECO:0000250|UniProtKB:Q91ZD4}.

Molecular Weight: 13.1 kDa Including tag.

UniProt: Q9ULK5

Pathways: WNT Signaling, Stem Cell Maintenance, Tube Formation, Asymmetric Protein Localization