VPS25 Protein (AA 1-176) (Strep Tag)

Catalog No. ABIN3075393

Product Details

Target: VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))

Protein Type: Recombinant

Protein Characteristics: AA 1-176

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This VPS25 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKSKSSFLIM WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TNGEDTEDEE FHGLDEATLL RALQALQQEH KAEIITVSDG RGVKFF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: VPS25 (Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))

Alternative Name: VPS25 (VPS25 Products)

Synonyms: VPS25 Protein, CG14750 Protein, Dmel\\CG14750 Protein, ESCRT-I Protein, ESCRT-II Protein, dVps25 Protein, l(2)44Db Protein, l(2)k08904 Protein, vps25 Protein, GB16268 Protein, derp9 Protein, eap20 Protein, fap20 Protein, DDBDRAFT_0189483 Protein, DDBDRAFT_0234026 Protein, DDB_0189483 Protein, DDB_0234026 Protein, 1110020N13Rik Protein, AW107467 Protein, D11Wsu68e Protein, im:6898134 Protein, zgc:162560 Protein, DERP9 Protein, EAP20 Protein, FAP20 Protein, vacuolar protein sorting 25 homolog Protein, vacuolar protein sorting 25 homolog (S. cerevisiae) pseudogene Protein, vacuolar protein sorting-associated protein 25 Protein, WNK lysine deficient protein kinase 4 Protein, Vacuolar protein sorting 25 Protein, vacuolar protein-sorting-associated protein 25 Protein, vacuolar protein sorting 25 homolog L homeolog Protein, vacuolar protein sorting 25 Protein, vacuolar protein sorting 25 homolog (S. cerevisiae) Protein, E2F/DP family winged-helix DNA-binding domain-containing protein Protein, VPS25 Protein, LOC459431 Protein, CpipJ_CPIJ010292 Protein, WNK4 Protein, Vps25 Protein, LOC412381 Protein, vps25.L Protein, vps25 Protein

Background: Vacuolar protein-sorting-associated protein 25 (hVps25) (Dermal papilla-derived protein 9) (ELL-associated protein of 20 kDa) (ESCRT-II complex subunit VPS25),FUNCTION: Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses. {ECO:0000269|PubMed:18723511}.

Molecular Weight: 20.7 kDa

UniProt: Q9BRG1