WDR20 Protein (AA 2-569) (His tag,GST tag)

Catalog No. ABIN3075585

Product Details

Target: WDR20 (WD Repeat Domain 20 (WDR20))

Protein Type: Recombinant

Protein Characteristics: AA 2-569

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This WDR20 protein is labelled with His tag,GST tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSATEG GGKEMNEIKT QFTTREGLYK LLPHSEYSRP NRVPFNSQGS NPVRVSFVNL NDQSGNGDRL CFNVGRELYF YIYKGVRKAA DLSKPIDKRI YKGTQPTCHD FNHLTATAES VSLLVGFSAG QVQLIDPIKK ETSKLFNEER LIDKSRVTCV KWVPGSESLF LVAHSSGNMY LYNVEHTCGT TAPHYQLLKQ GESFAVHTCK SKSTRNPLLK WTVGEGALNE FAFSPDGKFL ACVSQDGFLR VFNFDSVELH GTMKSYFGGL LCVCWSPDGK YIVTGGEDDL VTVWSFVDCR VIARGHGHKS WVSVVAFDPY TTSVEEGDPM EFSGSDEDFQ DLLHFGRDRA NSTQSRLSKR NSTDSRPVSV TYRFGSVGQD TQLCLWDLTE DILFPHQPLS RARTHTNVMN ATSPPAGSNG NSVTTPGNSV PPPLPRSNSL PHSAVSNAGS KSSVMDGAIA SGVSKFATLS LHDRKERHHE KDHKRNHSMG HISSKSSDKL NLVTKTKTDP AKTLGTPLCP RMEDVPLLEP LICKKIAHER LTVLIFLEDC IVTACQEGFI CTWGRPGKVV SFNPHHHHHH
Sequence including N-terminal GST tag and C-terminal His-tag.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human WDR20 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).
• Tag Location: N-terminal GST tag, C-terminal His Tag

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using EDTA resistant His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: WDR20 (WD Repeat Domain 20 (WDR20))

Alternative Name: WDR20 (WDR20 Products)

Synonyms: DMR Protein, 2310040A13Rik Protein, AI549910 Protein, WDR20 Protein, Wdr20 Protein, wdr20 Protein, WD repeat domain 20 Protein, WD repeat domain 20a Protein, WD repeat domain 20 S homeolog Protein, WD repeat domain 20b Protein, WDR20 Protein, Wdr20 Protein, wdr20 Protein, wdr20a Protein, wdr20.S Protein, wdr20b Protein

Molecular Weight: 63.7 kDa Including tag.

UniProt: Q8TBZ3