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STRN3 Protein (AA 1-797) (Strep Tag)

Crystallography grade STRN3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Catalog No. ABIN3077422
  • Target See all STRN3 Proteins
    STRN3 (Striatin, Calmodulin Binding Protein 3 (STRN3))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-797
    Origin
    • 1
    • 1
    • 1
    Human
    Source
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This STRN3 protein is labelled with Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequence
    MDELAGGGGG GPGMAAPPRQ QQGPGGNLGL SPGGNGAAGG GGPPASEGAG PAAGPELSRP QQYTIPGILH YIQHEWARFE MERAHWEVER AELQARIAFL QGERKGQENL KKDLVRRIKM LEYALKQERA KYHKLKYGTE LNQGDLKMPT FESEETKDTE APTAPQNSQL TWKQGRQLLR QYLQEVGYTD TILDVRSQRV RSLLGLSNSE PNGSVETKNL EQILNGGESP KQKGQEIKRS SGDVLETFNF LENADDSDED EENDMIEGIP EGKDKHRMNK HKIGNEGLAA DLTDDPDTEE ALKEFDFLVT AEDGEGAGEA RSSGDGTEWD KDDLSPTAEV WDVDQGLISK LKEQYKKERK GKKGVKRANR TKLYDMIADL GDDELPHIPS GIINQSRSAS TRMTDHEGAR AEEAEPITFP SGGGKSFIMG SDDVLLSVLG LGDLADLTVT NDADYSYDLP ANKDAFRKTW NPKYTLRSHF DGVRALAFHP VEPVLVTASE DHTLKLWNLQ KTVPAKKSAS LDVEPIYTFR AHIGPVLSLA ISSNGEQCFS GGIDATIQWW NMPSPSVDPY DTYEPNVLAG TLVGHTDAVW GLAYSGIKNQ LLSCSADGTV RLWNPQEKLP CICTYNGDKK HGIPTSVDFI GCDPAHMVTS FNTGSAVIYD LETSQSLVIL SSQVDSGLQS NNHINRVVSH PTLPVTITAH EDRHIKFFDN KTGKMIHSMV AHLDAVTSLA VDPNGIYLMS GSHDCSIRLW NLDSKTCVQE ITAHRKKLDE SIYDVAFHSS KAYIASAGAD ALAKVFV
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Grade
    Crystallography grade
    Top Product
    Discover our top product STRN3 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    STRN3 (Striatin, Calmodulin Binding Protein 3 (STRN3))
    Alternative Name
    STRN3 (STRN3 Products)
    Synonyms
    STRN3 Protein, GS2NA Protein, KIAA1333 Protein, MGC175239 Protein, id:ibd1241 Protein, wu:fc02f06 Protein, si:dz115o7.2 Protein, si:dz234g15.7 Protein, si:busm1-115o7.2 Protein, MGC145300 Protein, strn Protein, MGC80575 Protein, SG2NA Protein, Gs2na Protein, Sg2na Protein, striatin 3 Protein, striatin, calmodulin binding protein 3 Protein, striatin-3 Protein, striatin 3 L homeolog Protein, STRN3 Protein, strn3 Protein, LOC100180000 Protein, strn3.L Protein, Strn3 Protein
    Background
    Striatin-3 (Cell cycle autoantigen SG2NA) (S/G2 antigen),FUNCTION: Binds calmodulin in a calcium dependent manner. May function as scaffolding or signaling protein.
    Molecular Weight
    87.2 kDa
    UniProt
    Q13033
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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