Choline Kinase alpha Protein (CHKA) (AA 1-457) (His tag)
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- Target See all Choline Kinase alpha (CHKA) Proteins
- Choline Kinase alpha (CHKA)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-457
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This Choline Kinase alpha protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG QQPPLALPPP PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP GAWRGLREDE FHISVIRGGL SNMLFQCSLP DTTATLGDEP RKVLLRLYGA ILQMRSCNKE GSEQAQKENE FQGAEAMVLE SVMFAILAER SLGPKLYGIF PQGRLEQFIP SRRLDTEELS LPDISAEIAE KMATFHGMKM PFNKEPKWLF GTMEKYLKEV LRIKFTEESR IKKLHKLLSY NLPLELENLR SLLESTPSPV VFCHNDCQEG NILLLEGREN SEKQKLMLID FEYSSYNYRG FDIGNHFCEW MYDYSYEKYP FFRANIRKYP TKKQQLHFIS SYLPAFQNDF ENLSTEEKSI IKEEMLLEVN RFALASHFLW GLWSIVQAKI SSIEFGYMDY AQARFDAYFH QKRKLGV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CHKA Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product CHKA Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Choline Kinase alpha (CHKA)
- Alternative Name
- CHKA (CHKA Products)
- Synonyms
- im:7143625 Protein, wu:fd46h01 Protein, si:dkey-12e7.3 Protein, CHKA Protein, chkb Protein, ATCK1 Protein, CHOLINE KINASE Protein, CK Protein, F14O23.8 Protein, F14O23_8 Protein, choline kinase 1 Protein, CHK Protein, CKI Protein, EK Protein, CK/EK-alpha Protein, Chetk-alpha Protein, Chk Protein, ChoK Protein, EtnK-alpha Protein, CK-R Protein, choline kinase alpha Protein, choline kinase Protein, choline kinase 1 Protein, chka Protein, CHKA Protein, MCYG_00090 Protein, PF14_0020 Protein, CK1 Protein, CNI01400 Protein, Chka Protein
- Background
- Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. {ECO:0000269|PubMed:19915674}.
- Molecular Weight
- 53.2 kDa Including tag.
- UniProt
- P35790
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