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CAMK2N1 Protein (AA 1-78) (Strep Tag)

Crystallography grade CAMK2N1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Catalog No. ABIN3077816
  • Target See all CAMK2N1 Proteins
    CAMK2N1 (Calcium/calmodulin-Dependent Protein Kinase II Inhibitor 1 (CAMK2N1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-78
    Origin
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 3
    • 2
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This CAMK2N1 protein is labelled with Strep Tag.
    Application
    SDS-PAGE (SDS), ELISA, Western Blotting (WB)
    Sequence
    MSEVLPYGDE KLSPYGDGGD VGQIFSCRLQ DTNNFFGAGQ NKRPPKLGQI GRSKRVVIED DRIDDVLKNM TDKAPPGV
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Grade
    Crystallography grade
    Top Product
    Discover our top product CAMK2N1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CAMK2N1 (Calcium/calmodulin-Dependent Protein Kinase II Inhibitor 1 (CAMK2N1))
    Alternative Name
    CAMK2N1 (CAMK2N1 Products)
    Synonyms
    camk2n1 Protein, fj49g09 Protein, wu:fj49g09 Protein, zgc:110291 Protein, 1810006K23Rik Protein, AI848599 Protein, AI891706 Protein, PRO1489 Protein, RP11-401M16.1 Protein, calcium/calmodulin-dependent protein kinase II inhibitor 1a Protein, calcium/calmodulin-dependent protein kinase II inhibitor 1 Protein, calcium/calmodulin dependent protein kinase II inhibitor 1 Protein, camk2n1a Protein, Camk2n1 Protein, CAMK2N1 Protein
    Background
    Calcium/calmodulin-dependent protein kinase II inhibitor 1 (CaMKII inhibitory protein alpha) (CaMKIIN-alpha),FUNCTION: Potent and specific inhibitor of CaM-kinase II (CAMK2) (By similarity). Plays a role in the maintenance of long-term retrieval-induced memory in response to contextual fear (By similarity). Modulates blood pressure and vascular reactivity via regulation of CAMK2 activity in addition to regulation of left ventricular mass (By similarity). Mediates the NLRP3 inflammasome in cardiomyocytes via acting as an inhibitor of the MAPK14/p38 and MAPK8/JNK pathways, thereby regulating ventricular remodeling and cardiac rhythm post-myocardial infarction (By similarity). Negatively effects insulin sensitivity and promotes lipid formation in adipose tissues independent of CAMK2 signaling (By similarity). {ECO:0000250|UniProtKB:Q6QWF9, ECO:0000250|UniProtKB:Q9JI15}.
    Molecular Weight
    8.6 kDa
    UniProt
    Q7Z7J9
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