Aromatase Protein (AA 1-503) (His tag)
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- Target See all Aromatase (CYP19A1) Proteins
- Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-503
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This Aromatase protein is labelled with His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Sequence
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MVLEMLNPIH YNITSIVPEA MPAATMPVLL LTGLFLLVWN YEGTSSIPGP GYCMGIGPLI SHGRFLWMGI GSACNYYNRV YGEFMRVWIS GEETLIISKS SSMFHIMKHN HYSSRFGSKL GLQCIGMHEK GIIFNNNPEL WKTTRPFFMK ALSGPGLVRM VTVCAESLKT HLDRLEEVTN ESGYVDVLTL LRRVMLDTSN TLFLRIPLDE SAIVVKIQGY FDAWQALLIK PDIFFKISWL YKKYEKSVKD LKDAIEVLIA EKRRRISTEE KLEECMDFAT ELILAEKRGD LTRENVNQCI LEMLIAAPDT MSVSLFFMLF LIAKHPNVEE AIIKEIQTVI GERDIKIDDI QKLKVMENFI YESMRYQPVV DLVMRKALED DVIDGYPVKK GTNIILNIGR MHRLEFFPKP NEFTLENFAK NVPYRYFQPF GFGPRGCAGK YIAMVMMKAI LVTLLRRFHV KTLQGQCVES IQKIHDLSLH PDETKNMLEM IFTPRNSDRC LEH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CYP19A1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product CYP19A1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))
- Alternative Name
- CYP19A1 (CYP19A1 Products)
- Synonyms
- ARO Protein, ARO1 Protein, CPV1 Protein, CYAR Protein, CYP19 Protein, CYPXIX Protein, P-450AROM Protein, Aromatase Protein, Cyp19 Protein, Cyp19a Protein, p450arom Protein, P450 arom Protein, aro Protein, cyp19 Protein, cyp19a Protein, p450arom-A Protein, P450AROM Protein, Ar Protein, ArKO Protein, Int-5 Protein, Int5 Protein, CYP19P1 Protein, CYP19A1 Protein, CYP19a1 Protein, P450aromA Protein, ar1 Protein, cypXIX Protein, etID309862.18 Protein, zgc:136541 Protein, AroB Protein, CYP19a2 Protein, P450aromB Protein, cyp19b Protein, zgc:92614 Protein, cyp19a1 Protein, cytochrome P450 family 19 subfamily A member 1 Protein, cytochrome P450, family 19, subfamily a, polypeptide 1 Protein, cytochrome P450 family 19 subfamily A member 1 L homeolog Protein, aromatase Protein, cytochrome P450 aromatase Protein, cytochrome P450, family 19, subfamily A, polypeptide 1 Protein, cytochrome P450 19A1 Protein, cytochrome P450, family 17, subfamily A, polypeptide 1 Protein, cytochrome P450, family 19 Protein, cytochrome P450, family 19, subfamily A, polypeptide 1a Protein, cytochrome P450, family 19, subfamily A, polypeptide 1b Protein, cytochrome P450 19A1-like Protein, CYP19A1 Protein, Cyp19a1 Protein, cyp19a1.L Protein, SAV_2383 Protein, pfcyp19b-tstr Protein, CYP19 Protein, cyp19a1a Protein, cyp19a1b Protein, LOC101174651 Protein
- Background
- Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
- Molecular Weight
- 58.8 kDa Including tag.
- UniProt
- P11511
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis
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