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Aromatase Protein (AA 1-503) (His tag)

Crystallography grade CYP19A1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3078303
  • Target See all Aromatase (CYP19A1) Proteins
    Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-503
    Origin
    • 4
    • 3
    • 2
    Human
    Source
    • 4
    • 3
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This Aromatase protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    MVLEMLNPIH YNITSIVPEA MPAATMPVLL LTGLFLLVWN YEGTSSIPGP GYCMGIGPLI SHGRFLWMGI GSACNYYNRV YGEFMRVWIS GEETLIISKS SSMFHIMKHN HYSSRFGSKL GLQCIGMHEK GIIFNNNPEL WKTTRPFFMK ALSGPGLVRM VTVCAESLKT HLDRLEEVTN ESGYVDVLTL LRRVMLDTSN TLFLRIPLDE SAIVVKIQGY FDAWQALLIK PDIFFKISWL YKKYEKSVKD LKDAIEVLIA EKRRRISTEE KLEECMDFAT ELILAEKRGD LTRENVNQCI LEMLIAAPDT MSVSLFFMLF LIAKHPNVEE AIIKEIQTVI GERDIKIDDI QKLKVMENFI YESMRYQPVV DLVMRKALED DVIDGYPVKK GTNIILNIGR MHRLEFFPKP NEFTLENFAK NVPYRYFQPF GFGPRGCAGK YIAMVMMKAI LVTLLRRFHV KTLQGQCVES IQKIHDLSLH PDETKNMLEM IFTPRNSDRC LEH
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CYP19A1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product CYP19A1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))
    Alternative Name
    CYP19A1 (CYP19A1 Products)
    Synonyms
    ARO Protein, ARO1 Protein, CPV1 Protein, CYAR Protein, CYP19 Protein, CYPXIX Protein, P-450AROM Protein, Aromatase Protein, Cyp19 Protein, Cyp19a Protein, p450arom Protein, P450 arom Protein, aro Protein, cyp19 Protein, cyp19a Protein, p450arom-A Protein, P450AROM Protein, Ar Protein, ArKO Protein, Int-5 Protein, Int5 Protein, CYP19P1 Protein, CYP19A1 Protein, CYP19a1 Protein, P450aromA Protein, ar1 Protein, cypXIX Protein, etID309862.18 Protein, zgc:136541 Protein, AroB Protein, CYP19a2 Protein, P450aromB Protein, cyp19b Protein, zgc:92614 Protein, cyp19a1 Protein, cytochrome P450 family 19 subfamily A member 1 Protein, cytochrome P450, family 19, subfamily a, polypeptide 1 Protein, cytochrome P450 family 19 subfamily A member 1 L homeolog Protein, aromatase Protein, cytochrome P450 aromatase Protein, cytochrome P450, family 19, subfamily A, polypeptide 1 Protein, cytochrome P450 19A1 Protein, cytochrome P450, family 17, subfamily A, polypeptide 1 Protein, cytochrome P450, family 19 Protein, cytochrome P450, family 19, subfamily A, polypeptide 1a Protein, cytochrome P450, family 19, subfamily A, polypeptide 1b Protein, cytochrome P450 19A1-like Protein, CYP19A1 Protein, Cyp19a1 Protein, cyp19a1.L Protein, SAV_2383 Protein, pfcyp19b-tstr Protein, CYP19 Protein, cyp19a1a Protein, cyp19a1b Protein, LOC101174651 Protein
    Background
    Catalyzes the formation of aromatic C18 estrogens from C19 androgens.
    Molecular Weight
    58.8 kDa Including tag.
    UniProt
    P11511
    Pathways
    Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis
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