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EBP Protein (AA 2-230) (rho-1D4 tag)

Crystallography grade EBP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3079342
  • Target See all EBP Proteins
    EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-230
    Origin
    • 2
    • 1
    Human
    Source
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This EBP protein is labelled with rho-1D4 tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    TTNAGPLHPY WPQHLRLDNF VPNDRPTWHI LAGLFSVTGV LVVTTWLLSG RAAVVPLGTW RRLSLCWFAV CGFIHLVIEG WFVLYYEDLL GDQAFLSQLW KEYAKGDSRY ILGDNFTVCM ETITACLWGP LSLWVVIAFL RQHPLRFILQ LVVSVGQIYG DVLYFLTEHR DGFQHGELGH PLYFWFYFVF MNALWLVLPG VLVLDAVKHL THAQSTLDAK ATKAKSKKN
    GSSGTETSQVAPA
    C-terminal Rho-tag
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human EBP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    The protein is purified from the cleared cell lysate using Rho1D4-tag capture materials. Eluate fractions are analyzed by SDS-PAGE.
    Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    in solution (20 mM Hepes, pH 7.5, 100 mM NaCl, 0.1 % DDM)
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    EBP (Emopamil Binding Protein (Sterol Isomerase) (EBP))
    Alternative Name
    EBP (EBP Products)
    Synonyms
    cdpx2 Protein, cho2 Protein, cpx Protein, cpxd Protein, zgc:91895 Protein, CDPX2 Protein, CHO2 Protein, CPX Protein, CPXD Protein, AI255399 Protein, Pabp Protein, Td Protein, mSI Protein, emopamil binding protein (sterol isomerase) Protein, emopamil binding protein (sterol isomerase) L homeolog Protein, phenylalkylamine Ca2+ antagonist (emopamil) binding protein Protein, Ebp Protein, ebp.L Protein, ebp Protein, EBP Protein
    Background
    Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers.
    Molecular Weight
    27.4 kDa Including tag.
    UniProt
    Q15125
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