HIBCH Protein (AA 33-386) (His tag)

Catalog No. ABIN3081417

Product Details

Target: HIBCH (3-hydroxyisobutyryl-CoA Hydrolase (HIBCH))

Protein Type: Recombinant

Protein Characteristics: AA 33-386

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This HIBCH protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: DAAEEVLLEK KGCTGVITLN RPKFLNALTL NMIRQIYPQL KKWEQDPETF LIIIKGAGGK AFCAGGDIRV ISEAEKAKQK IAPVFFREEY MLNNAVGSCQ KPYVALIHGI TMGGGVGLSV HGQFRVATEK CLFAMPETAI GLFPDVGGGY FLPRLQGKLG YFLALTGFRL KGRDVYRAGI ATHFVDSEKL AMLEEDLLAL KSPSKENIAS VLENYHTESK IDRDKSFILE EHMDKINSCF SANTVEEIIE NLQQDGSSFA LEQLKVINKM SPTSLKITLR QLMEGSSKTL QEVLTMEYRL SQACMRGHDF HEGVRAVLID KDQSPKWKPA DLKEVTEEDL NNHFKSLGSS DLKF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HIBCH Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: HIBCH (3-hydroxyisobutyryl-CoA Hydrolase (HIBCH))

Alternative Name: HIBCH (HIBCH Products)

Synonyms: DDBDRAFT_0189353 Protein, DDBDRAFT_0233831 Protein, DDB_0189353 Protein, DDB_0233831 Protein, T11J7.4 Protein, T11J7_4 Protein, T11J7.5 Protein, T11J7_5 Protein, HIBYLCOAH Protein, 2610509I15Rik Protein, AI648812 Protein, zgc:110824 Protein, 3-hydroxyisobutyryl-coenzyme A hydrolase Protein, 3-hydroxyisobutyryl-coenzyme a hydrolase,putative Protein, 3-hydroxyisobutyryl-Coenzyme A hydrolase Protein, 3-hydroxyisobutyryl-CoA hydrolase (predicted) Protein, 3-hydroxyisobutyryl-CoA hydrolase Protein, ATP-dependent caseinolytic (Clp) protease/crotonase family protein Protein, putative 3-hydroxyisobutyryl-coenzyme A hydrolase Protein, 3-hydroxyisobutyryl-CoA hydrolase L homeolog Protein, PVX_100950 Protein, CpipJ_CPIJ012030 Protein, PKH_145800 Protein, hibch Protein, MCYG_05070 Protein, SPBC2D10.09 Protein, CNA02940 Protein, LACBIDRAFT_292320 Protein, SJAG_00944 Protein, VDBG_07520 Protein, AT2G30650 Protein, AT2G30660 Protein, LBRM_32_3920 Protein, HIBCH Protein, Hibch Protein, hibch.L Protein

Background: Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA. {ECO:0000269|PubMed:8824301}.

Molecular Weight: 40.4 kDa Including tag.

UniProt: Q6NVY1

Pathways: Monocarboxylic Acid Catabolic Process