EIF5A Protein (AA 2-154) (His tag)

Catalog No. ABIN3081778

Product Details

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Protein Type: Recombinant

Protein Characteristics: AA 2-154

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This EIF5A protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: ADDLDFETGD AGASATFPMQ CSALRKNGFV VLKGRPCKIV EMSTSKTGKH GHAKVHLVGI DIFTGKKYED ICPSTHNMDV PNIKRNDFQL IGIQDGYLSL LQDSGEVRED LRLPEGDLGK EIEQKYDCGE EILITVLSAM TEEAAVAIKA MAK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EIF5A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Alternative Name: EIF5A (EIF5A Products)

Synonyms: EIF-5A Protein, EIF5A1 Protein, eIF5AI Protein, EIF5A Protein, zgc:55504 Protein, zgc:77429 Protein, wu:fb05b06 Protein, wu:fc23f04 Protein, wu:fc61c10 Protein, AA410058 Protein, D19Wsu54e Protein, Eif4d Protein, Eif5a1 Protein, eIF-4D Protein, eIF-5A Protein, eIF-5A-1 Protein, eIF-5A1 Protein, zgc:77099 Protein, wu:fb17e01 Protein, wu:fb63h04 Protein, wu:fk81e04 Protein, DDBDRAFT_0191442 Protein, DDBDRAFT_0218663 Protein, DDB_0191442 Protein, DDB_0218663 Protein, 1296 Protein, CG3186 Protein, Dmel\\CG3186 Protein, anon-EST:fe2H10 Protein, eIF5A Protein, eIF5a Protein, l(2)01296 Protein, EIF-5A1 Protein, NeIF-5A3 Protein, eif5a Protein, iff-2 Protein, eukaryotic translation initiation factor 5A Protein, eukaryotic translation initiation factor 5A2 Protein, eukaryotic translation initiation factor 5a Protein, putative eukaryotic initiation factor 5a Protein, CG3186 gene product from transcript CG3186-RB Protein, eukaryotic translation initiation factor 5A-1 Protein, eukaryotic translation initiation factor 5A L homeolog Protein, EIF5A Protein, eif5a2 Protein, Eif5a Protein, eif5a Protein, tif5A Protein, Tb11.03.0410 Protein, LOC5579111 Protein, EIF5A1 Protein, eIF-5A Protein, LOC107820748 Protein, eif5a.L Protein

Background: MRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts. {ECO:0000269|PubMed:15371445, ECO:0000269|PubMed:15452064, ECO:0000269|PubMed:16987817, ECO:0000269|PubMed:17187778, ECO:0000269|PubMed:17360499}.

Molecular Weight: 17.7 kDa Including tag.

UniProt: P63241

Pathways: Regulation of Muscle Cell Differentiation