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IPMK Protein (AA 2-416) (His tag)

Crystallography grade IPMK Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3081921
  • Target See all IPMK Proteins
    IPMK (Inositol Polyphosphate Multikinase (IPMK))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-416
    Origin
    • 3
    • 1
    • 1
    • 1
    Human
    Source
    • 2
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This IPMK protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    ATEPPSPLRV EAPGPPEMRT SPAIESTPEG TPQPAGGRLR FLNGCVPLSH QVAGHMYGKD KVGILQHPDG TVLKQLQPPP RGPRELEFYN MVYAADCFDG VLLELRKYLP KYYGIWSPPT APNDLYLKLE DVTHKFNKPC IMDVKIGQKS YDPFASSEKI QQQVSKYPLM EEIGFLVLGM RVYHVHSDSY ETENQHYGRS LTKETIKDGV SRFFHNGYCL RKDAVAASIQ KIEKILQWFE NQKQLNFYAS SLLFVYEGSS QPTTTKLNDR TLAEKFLSKG QLSDTEVLEY NNNFHVLSST ANGKIESSVG KSLSKMYARH RKIYTKKHHS QTSLKVENLE QDNGWKSMSQ EHLNGNVLSQ LEKVFYHLPT GCQEIAEVEV RMIDFAHVFP SNTIDEGYVY GLKHLISVLR SILDN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human IPMK Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product IPMK Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    IPMK (Inositol Polyphosphate Multikinase (IPMK))
    Alternative Name
    IPMK (IPMK Products)
    Synonyms
    2410017C19Rik Protein, AA408208 Protein, Impk Protein, MGC83832 Protein, IPMK Protein, zgc:158331 Protein, ipmk Protein, si:dkey-223l5.1 Protein, Ipk2 Protein, ARABIDOPSIS THALIANA INOSITOL POLYPHOSPHATE KINASE 2 BETA Protein, ATIPK2BETA Protein, AtIpk2-beta Protein, AtIpk2beta Protein, INOSITOL POLYPHOSPHATE KINASE 2 BETA Protein, IPK2B Protein, MAC9.7 Protein, MAC9_7 Protein, inositol polyphosphate kinase 2 beta Protein, inositol polyphosphate multikinase Protein, inositol polyphosphate multikinase L homeolog Protein, inositol polyphosphate multikinase b Protein, inositol polyphosphate multikinase a Protein, inositol polyphosphate kinase 2 beta Protein, IPMK Protein, Ipmk Protein, ipmk.L Protein, ipmkb Protein, EDI_171250 Protein, CC1G_06147 Protein, CpipJ_CPIJ006477 Protein, CpipJ_CPIJ016779 Protein, PAAG_02384 Protein, MCYG_03683 Protein, ipmka Protein, EDI_070480 Protein, VDBG_01275 Protein, MGYG_03269 Protein, Tsp_05937 Protein, IPK2BETA Protein
    Background
    Inositol phosphate kinase with a broad substrate specificity. Has a preference for inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and inositol 1,3,4,6-tetrakisphosphate (Ins(1,3,4,6)P4). {ECO:0000269|PubMed:12027805, ECO:0000269|PubMed:12223481}.
    Molecular Weight
    48.0 kDa Including tag.
    UniProt
    Q8NFU5
    Pathways
    Tube Formation
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