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MRGPRX3 Protein (AA 1-322) (rho-1D4 tag)

Crystallography grade MRGPRX3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3083859
  • Target See all MRGPRX3 Proteins
    MRGPRX3 (MAS-Related GPR, Member X3 (MRGPRX3))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-322
    Origin
    Human
    Source
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This MRGPRX3 protein is labelled with rho-1D4 tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MDSTIPVLGT ELTPINGREE TPCYKQTLSF TGLTCIVSLV ALTGNAVVLW LLGCRMRRNA VSIYILNLVA ADFLFLSGHI ICSPLRLINI RHPISKILSP VMTFPYFIGL SMLSAISTER CLSILWPIWY HCRRPRYLSS VMCVLLWALS LLRSILEWMF CDFLFSGANS VWCETSDFIT IAWLVFLCVV LCGSSLVLLV RILCGSRKMP LTRLYVTILL TVLVFLLCGL PFGIQWALFS RIHLDWKVLF CHVHLVSIFL SALNSSANPI IYFFVGSFRQ RQNRQNLKLV LQRALQDTPE VDEGGGWLPQ ETLELSGSRL EQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human MRGPRX3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product MRGPRX3 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    MRGPRX3 (MAS-Related GPR, Member X3 (MRGPRX3))
    Alternative Name
    MRGPRX3 (MRGPRX3 Products)
    Synonyms
    Gm660 Protein, Mrgprx3 Protein, GPCR Protein, MRGX3 Protein, SNSR1 Protein, SNSR2 Protein, MrgA Protein, Mrga10 Protein, Mrgpra Protein, MAS-related GPR, member X3, pseudogene Protein, MAS related GPR family member X3 Protein, mas-related G-protein coupled receptor member X2-like Protein, MAS-related GPR, member X3 Protein, Mrgprx3-ps Protein, MRGPRX3 Protein, LOC100359273 Protein, Mrgprx3 Protein
    Background
    Orphan receptor. Probably involved in the function of nociceptive neurons. May regulate nociceptor function and/or development, including the sensation or modulation of pain. Potently activated by enkephalins (By similarity). {ECO:0000250}.
    Molecular Weight
    37.7 kDa Including tag.
    UniProt
    Q96LB0
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