Nucleosome Assembly Protein 1-Like 3 (NAP1L3) (AA 1-506) protein (His tag)

Details for Product No. ABIN3084773, Supplier: Log in to see
Protein Name
  • MB20
  • NPL3
  • nucleosome assembly protein 1-like 3
  • NAP1L3
  • LOC100351950
  • Nap1l3
Protein Characteristics
AA 1-506
1
1
1
1
Origin
Human
1
1
1
Source
Insect Cells
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This NAP1L3 protein is labelled with His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Available images

Sequence MAEADFKMVS EPVAHGVAEE EMASSTSDSG EESDSSSSSS STSDSSSSSS TSGSSSGSGS SSSSSGSTSS RSRLYRKKRV PEPSRRARRA PLGTNFVDRL PQAVRNRVQA LRNIQDECDK VDTLFLKAIH DLERKYAELN KPLYDRRFQI INAEYEPTEE ECEWNSEDEE FSSDEEVQDN TPSEMPPLEG EEEENPKENP EVKAEEKEVP KEIPEVKDEE KEVPKEIPEV KAEEKADSKD CMEATPEVKE DPKEVPQVKA DDKEQPKATE AKARAAVRET HKRVPEERLQ DSVDLKRARK GKPKREDPKG IPDYWLIVLK NVDKLGPMIQ KYDEPILKFL SDVSLKFSKP GQPVSYTFEF HFLPNPYFRN EVLVKTYIIK AKPDHNDPFF SWGWEIEDCK GCKIDWRRGK DVTVTTTQSR TTATGEIEIQ PRVVPNASFF NFFSPPEIPM IGKLEPREDA ILDEDFEIGQ ILHDNVILKS IYYYTGEVNG TYYQFGKHYG NKKYRK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human NAP1L3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Molecular Weight 58.5 kDa Including tag.
UniProt Q99457
Research Area Chromatin and Nuclear Signaling
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Comment

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Nucleosome Assembly Protein 1-Like 3 (NAP1L3) (AA 1-506) protein (His tag) (ABIN3084773) „Crystallography Grade“ protein due to multi-step, protein-specific purification process