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PEPCK Protein (AA 33-640) (His tag)

Crystallography grade PEPCK Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3085613
  • Target See all PEPCK Proteins
    PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 33-640
    Origin
    • 3
    • 1
    Human
    Source
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This PEPCK protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    LSGDLGQLPT GIRDFVEHSA RLCQPEGIHI CDGTEAENTA TLTLLEQQGL IRKLPKYNNC WLARTDPKDV ARVESKTVIV TPSQRDTVQL PPGGARGQLG NWMSPADFQR AVDERFPGCM QGRTMYVLPF SMGPVGSPLS RIGVQLTDSA YVVASMRIMT RLGTPVLQAL GDGDFVKCLH SVGQPLTGQG EPVSQWPCNP EKTLIGHVPD QREIISFGSG YGGNSLLGKK CFALRIASRL ARDEGWLAEH MLILGITSPA GKKRYVAAAF PSACGKTNLA MMRPALPGWK VECVGDDIAW MRFDSEGRLR AINPENGFFG VAPGTSATTN PNAMATIQSN TIFTNVAETS DGGVYWEGID QPLPPGVTVT SWLGKPWKPG DKEPCAHPNS RFCAPARQCP IMDPAWEAPE GVPIDAIIFG GRRPKGVPLV YEAFNWRHGV FVGSAMRSES TAAAEHKGKI IMHDPFAMRP FFGYNFGHYL EHWLSMEGRK GAQLPRIFHV NWFRRDEAGH FLWPGFGENA RVLDWICRRL EGEDSARETP IGLVPKEGAL DLSGLRAIDT TQLFSLPKDF WEQEVRDIRS YLTEQVNQDL PKEVLAELEA LERRVHKM
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PCK2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product PEPCK Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
    Alternative Name
    PCK2 (PEPCK Products)
    Synonyms
    PEPCK Protein, PEPCK-M Protein, PEPCK2 Protein, 1810010O14Rik Protein, 9130022B02Rik Protein, pck Protein, zgc:77867 Protein, id:ibd5157 Protein, pck2 Protein, pck2.S Protein, phosphoenolpyruvate carboxykinase 2, mitochondrial Protein, phosphoenolpyruvate carboxykinase 2 (mitochondrial) Protein, phosphoenolpyruvate carboxykinase 2 (mitochondrial) L homeolog Protein, PCK2 Protein, Pck2 Protein, pck2 Protein, pck2.L Protein
    Background
    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. {ECO:0000250}.
    Molecular Weight
    68.1 kDa Including tag.
    UniProt
    Q16822
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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