PSMD8 Protein (AA 1-350) (Strep Tag)

Catalog No. ABIN3086673

Product Details

Target: PSMD8 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8))

Protein Type: Recombinant

Protein Characteristics: AA 1-350

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PSMD8 protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MFIKGRAPRA PPRERRRATR GGLRQVVAPP RALGSTSRPH FRRASVCRRR CRKSGGLLAA SRKMAAAAVN GAAGFSSSGP AATSGAVLQA ATGMYEQLKG EWNRKSPNLS KCGEELGRLK LVLLELNFLP TTGTKLTKQQ LILARDILEI GAQWSILRKD IPSFERYMAQ LKCYYFDYKE QLPESAYMHQ LLGLNLLFLL SQNRVAEFHT ELERLPAKDI QTNVYIKHPV SLEQYLMEGS YNKVFLAKGN IPAESYTFFI DILLDTIRDE IAGCIEKAYE KILFTEATRI LFFNTPKKMT DYAKKRGWVL GPNNYYSFAS QQQKPEDTTI PSTELAKQVI EYARQLEMIV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PSMD8 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 8 (PSMD8))

Alternative Name: PSMD8 (PSMD8 Products)

Synonyms: HIP6 Protein, HYPF Protein, Nin1p Protein, Rpn12 Protein, S14 Protein, p31 Protein, hip6 Protein, hypf Protein, nin1p Protein, rpn12 Protein, psmd8b Protein, 6720456J22Rik Protein, AA407360 Protein, AL033291 Protein, AL033322 Protein, AL033323 Protein, C76433 Protein, psmd8 Protein, psmd8a Protein, fa93g07 Protein, fb17c09 Protein, fb49a10 Protein, zgc:86762 Protein, wu:fa93g07 Protein, wu:fb17c09 Protein, wu:fb49a10 Protein, PSMD8 Protein, proteasome 26S subunit, non-ATPase 8 Protein, proteasome 26S subunit, non-ATPase 8 S homeolog Protein, proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 Protein, proteasome 26S subunit, non-ATPase 8 L homeolog Protein, proteasome (prosome, macropain) 26S subunit, non-ATPase, 8 pseudogene Protein, PSMD8 Protein, psmd8.S Protein, Psmd8 Protein, psmd8.L Protein, psmd8 Protein, LOC468490 Protein

Background: 26S proteasome non-ATPase regulatory subunit 8 (26S proteasome regulatory subunit RPN12) (26S proteasome regulatory subunit S14) (p31),FUNCTION: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. {ECO:0000269|PubMed:1317798}.

Molecular Weight: 39.6 kDa

UniProt: P48556

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Proton Transport, Synthesis of DNA, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway