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UQCRH Protein (AA 14-91) (His tag)

Crystallography grade Uqcrh Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Catalog No. ABIN3086889
  • Target See all UQCRH (Uqcrh) Proteins
    UQCRH (Uqcrh) (Ubiquinol-Cytochrome C Reductase Hinge Protein (Uqcrh))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 14-91
    Origin
    • 5
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 3
    • 3
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This UQCRH protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequence
    GDPEEEEEEE EELVDPLTTV REQCEQLEKC VKARERLELC DERVSSRSHT EEDCTEELFD FLHARDHCVA HKLFNNLK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human UQCRH Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product Uqcrh Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    UQCRH (Uqcrh) (Ubiquinol-Cytochrome C Reductase Hinge Protein (Uqcrh))
    Alternative Name
    UQCRH (Uqcrh Products)
    Synonyms
    QCR6 Protein, UQCR8 Protein, im:7141026 Protein, zgc:123330 Protein, UQCRH Protein, 2210416J04Rik Protein, 2310021J10Rik Protein, 2610041P16Rik Protein, DDBDRAFT_0203632 Protein, DDBDRAFT_0233077 Protein, DDB_0203632 Protein, DDB_0233077 Protein, ubiquinol-cytochrome c reductase hinge protein Protein, cytochrome b-c1 complex subunit 6, mitochondrial Protein, ubiquinol-cytochrome c reductase hinge protein pseudogene Protein, ubiquinol-cytochrome-c reductase hinge protein Protein, ubiquinol-cytochrome C reductase hinge protein Protein, Ubiquinol-cytochrome C reductase hinge protein Protein, ubiquinol-cytochrome c reductase hinge protein,putative Protein, subunit of the ubiquinol-cytochrome c reductase complex, putative Protein, UQCRH Protein, uqcrh Protein, LOC742376 Protein, LOC619515 Protein, LOC701662 Protein, Uqcrh Protein, LOC456466 Protein, TP04_0347 Protein, PVX_085190 Protein, Bm1_14750 Protein, PKH_132350 Protein, CD36_84010 Protein, Tsp_07393 Protein, LOC102136267 Protein
    Background
    This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.
    Molecular Weight
    10.2 kDa Including tag.
    UniProt
    P07919
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