SLC22A13 Protein (AA 1-551) (Strep Tag)

Catalog No. ABIN3087648

Product Details

Target: SLC22A13 (Solute Carrier Family 22 (Organic Anion Transporter), Member 13 (SLC22A13))

Protein Type: Recombinant

Protein Characteristics: AA 1-551

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC22A13 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAQFVQVLAE IGDFGRFQIQ LLILLCVLNF LSPFYFFAHV FMVLDEPHHC AVAWVKNHTF NLSAAEQLVL SVPLDTAGHP EPCLMFRPPP ANASLQDILS HRFNETQPCD MGWEYPENRL PSLKNEFNLV CDRKHLKDTT QSVFMAGLLV GTLMFGPLCD RIGRKATILA QLLLFTLIGL ATAFVPSFEL YMALRFAVAT AVAGLSFSNV TLLTEWVGPS WRTQAVVLAQ CNFSLGQMVL AGLAYGFRNW RLLQITGTAP GLLLFFYFWA LPESARWLLT RGRMDEAIQL IQKAASVNRR KLSPELMNQL VPEKTGPSGN ALDLFRHPQL RKVTLIIFCV WFVDSLGYYG LSLQVGDFGL DVYLTQLIFG AVEVPARCSS IFMMQRFGRK WSQLGTLVLG GLMCIIIIFI PADLPVVVTM LAVVGKMATA AAFTISYVYS AELFPTILRQ TGMGLVGIFS RIGGILTPLV ILLGEYHAAL PMLIYGSLPI VAGLLCTLLP ETHGQGLKDT LQDLELGPHP RSPKSVPSEK ETEAKGRTSS PGVAFVSSTY F
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC22A13 (Solute Carrier Family 22 (Organic Anion Transporter), Member 13 (SLC22A13))

Alternative Name: SLC22A13 (SLC22A13 Products)

Synonyms: OAT10 Protein, OCTL1 Protein, OCTL3 Protein, ORCTL-3 Protein, ORCTL3 Protein, AI648912 Protein, solute carrier family 22 member 13 Protein, solute carrier family 22 (organic cation transporter), member 13 Protein, SLC22A13 Protein, Slc22a13 Protein

Background: Solute carrier family 22 member 13 (Organic anion transporter 10) (OAT10) (Organic cation transporter-like 3) (ORCTL-3) (ORCTL3),FUNCTION: Anion antiporter that mediates the transport of urate, orotate and nicotinate in exchange for organic or inorganic anions (PubMed:18411268, PubMed:35144162, PubMed:31780526, PubMed:35462902). Translocates urate and orotate across the apical membrane of proximal tubule epithelial cells and involved in urate renal reabsorption (PubMed:18411268, PubMed:35144162, PubMed:31780526). Possibly involved in orotate renal reabsorption and nicotinate intestinal reabsorption (PubMed:18411268, PubMed:35144162). Mediates urate uptake by an exchange with organic anions such as (S)-lactate, succinate, glutathione and nicotinate (PubMed:18411268). Urate and orotate transports are Cl(-)-dependent (PubMed:35144162, PubMed:35462902). Shows similar transport characteristics as the urate/orotate renal antiporter SLC22A12/URAT1 and may act as a compensator of SLC22A12/URAT1 in certain conditions (Probable). {ECO:0000269|PubMed:18411268, ECO:0000269|PubMed:31780526, ECO:0000269|PubMed:35144162, ECO:0000269|PubMed:35462902, ECO:0000305|PubMed:35144162, ECO:0000305|PubMed:35462902}.

Molecular Weight: 60.9 kDa

UniProt: Q9Y226