HSD3B1 Protein (AA 2-373) (rho-1D4 tag)

Catalog No. ABIN3087931

Product Details

Target: HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))

Protein Type: Recombinant

Protein Characteristics: AA 2-373

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This HSD3B1 protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: TGWSCLVTGA GGFLGQRIIR LLVKEKELKE IRVLDKAFGP ELREEFSKLQ NKTKLTVLEG DILDEPFLKR ACQDVSVIIH TACIIDVFGV THRESIMNVN VKGTQLLLEA CVQASVPVFI YTSSIEVAGP NSYKEIIQNG HEEEPLENTW PAPYPHSKKL AEKAVLAANG WNLKNGGTLY TCALRPMYIY GEGSRFLSAS INEALNNNGI LSSVGKFSTV NPVYVGNVAW AHILALRALQ DPKKAPSIRG QFYYISDDTP HQSYDNLNYT LSKEFGLRLD SRWSFPLSLM YWIGFLLEIV SFLLRPIYTY RPPFNRHIVT LSNSVFTFSY KKAQRDLAYK PLYSWEEAKQ KTVEWVGSLV DRHKETLKSK TQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HSD3B1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))

Alternative Name: HSD3B1 (HSD3B1 Products)

Synonyms: 3BETAHSD Protein, HSD3B Protein, HSDB3 Protein, HSDB3A Protein, I Protein, SDR11E1 Protein, HSD3B1 Protein, MGC131206 Protein, D3Ertd383e Protein, 3B-HSD Protein, 3BHSD Protein, si:rp71-68n21.10 Protein, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 Protein, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 Protein, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 L homeolog Protein, HSD3B1 Protein, LOC469446 Protein, LOC713091 Protein, hsd3b1.L Protein, LOC100451655 Protein, Hsd3b1 Protein, hsd3b1 Protein

Background: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone. {ECO:0000269|PubMed:1401999, ECO:0000269|PubMed:2139411}.

Molecular Weight: 43.3 kDa Including tag.

UniProt: P14060

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis