ABCA4 Protein (AA 1398-1727) (His tag)

Catalog No. ABIN3087963

Product Details

Target: ABCA4 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 4 (ABCA4))

Protein Type: Recombinant

Protein Characteristics: AA 1398-1727

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This ABCA4 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: GEYPALTLHP WIYGQQYTFF SMDEPGSEQF TVLADVLLNK PGFGNRCLKE GWLPEYPCGN STPWKTPSVS PNITQLFQKQ KWTQVNPSPS CRCSTREKLT MLPECPEGAG GLPPPQRTQR STEILQDLTD RNISDFLVKT YPALIRSSLK SKFWVNEQRY GGISIGGKLP VVPITGEALV GFLSDLGRIM NVSGGPITRE ASKEIPDFLK HLETEDNIKV WFNNKGWHAL VSFLNVAHNA ILRASLPKDR SPEEYGITVI SQPLNLTKEQ LSEITVLTTS VDAVVAICVI FSMSFVPASF VLYLIQERVN KSKHLQFISG VSPTTYWVTN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ABCA4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ABCA4 (ATP-Binding Cassette, Sub-Family A (ABC1), Member 4 (ABCA4))

Alternative Name: ABCA4 (ABCA4 Products)

Synonyms: ABC10 Protein, ABCR Protein, ARMD2 Protein, CORD3 Protein, FFM Protein, RMP Protein, RP19 Protein, STGD Protein, STGD1 Protein, AW050280 Protein, Abc10 Protein, Abcr Protein, D430003I15Rik Protein, RmP Protein, abcr Protein, ffm Protein, rmp Protein, rp19 Protein, stgd Protein, abc10 Protein, armd2 Protein, cord3 Protein, stgd1 Protein, zgc:91823 Protein, ATP binding cassette subfamily A member 4 Protein, ATP-binding cassette, sub-family A (ABC1), member 4 Protein, ATP binding cassette subfamily A member 4 L homeolog Protein, ATP-binding cassette, sub-family A (ABC1), member 4a Protein, ABCA4 Protein, Abca4 Protein, abca4 Protein, abca4.L Protein, abca4a Protein

Background: In the visual cycle, acts as an inward-directed retinoid flipase, retinoid substrates imported by ABCA4 from the extracellular or intradiscal (rod) membrane surfaces to the cytoplasmic membrane surface are all-trans-retinaldehyde (ATR) and N-retinyl-phosphatidyl-ethanolamine (NR-PE). Once transported to the cytoplasmic surface, ATR is reduced to vitamin A by trans-retinol dehydrogenase (tRDH) and then transferred to the retinal pigment epithelium (RPE) where it is converted to 11-cis-retinal. May play a role in photoresponse, removing ATR/NR-PE from the extracellular photoreceptor surfaces during bleach recovery. {ECO:0000269|PubMed:10075733}.

Molecular Weight: 37.8 kDa Including tag.

UniProt: P78363