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ABCC9 Protein (AA 1267-1549) (His tag)

Crystallography grade ABCC9 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Catalog No. ABIN3088039
  • Target See all ABCC9 Proteins
    ABCC9 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 9 (ABCC9))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1267-1549
    Origin
    • 4
    • 4
    Human
    Source
    • 5
    • 3
    Insect Cells
    Purification tag / Conjugate
    This ABCC9 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Sequence
    RNLADLEVQM GAVKKVNSFL TMESENYEGT MDPSQVPEHW PQEGEIKIHD LCVRYENNLK PVLKHVKAYI KPGQKVGICG RTGSGKSSLS LAFFRMVDIF DGKIVIDGID ISKLPLHTLR SRLSIILQDP ILFSGSIRFN LDPECKCTDD RLWEALEIAQ LKNMVKSLPG GLDAVVTEGG ENFSVGQRQL FCLARAFVRK SSILIMDEAT ASIDMATENI LQKVVMTAFA DRTVVTIAHR VSSIMDAGLV LVFSEGILVE CDTVPNLLAH KNGLFSTLVM TNK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ABCC9 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ABCC9 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ABCC9 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 9 (ABCC9))
    Alternative Name
    ABCC9 (ABCC9 Products)
    Synonyms
    SUR2B Protein, DDBDRAFT_0215814 Protein, DDBDRAFT_0216237 Protein, DDB_0215814 Protein, DDB_0216237 Protein, si:dkey-183c2.3 Protein, sur2 Protein, ABC37 Protein, ATFB12 Protein, CANTU Protein, CMD1O Protein, SUR2 Protein, SUR2A Protein, AI414027 Protein, AI449286 Protein, Sur2 Protein, ABCC9 Protein, ATP binding cassette subfamily C member 9 Protein, ATP-binding cassette sub-family C member 8 Protein, ABC transporter C family protein Protein, ATP-binding cassette sub-family C member 9 Protein, ATP-binding cassette, sub-family C (CFTR/MRP), member 9 Protein, ABCC9 Protein, LOC581821 Protein, abcC9 Protein, LOC100470981 Protein, abcc9 Protein, Abcc9 Protein
    Background
    Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with KCNJ11. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation. {ECO:0000269|PubMed:9831708}.
    Molecular Weight
    32.2 kDa Including tag.
    UniProt
    O60706
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