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ACTR1A Protein (AA 1-376) (His tag)

Crystallography grade ACTR1A Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3088295
  • Target See all ACTR1A Proteins
    ACTR1A (Alpha Centractin (ACTR1A))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-376
    Origin
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 5
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This ACTR1A protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MESYDVIANQ PVVIDNGSGV IKAGFAGDQI PKYCFPNYVG RPKHVRVMAG ALEGDIFIGP KAEEHRGLLS IRYPMEHGIV KDWNDMERIW QYVYSKDQLQ TFSEEHPVLL TEAPLNPRKN RERAAEVFFE TFNVPALFIS MQAVLSLYAT GRTTGVVLDS GDGVTHAVPI YEGFAMPHSI MRIDIAGRDV SRFLRLYLRK EGYDFHSSSE FEIVKAIKER ACYLSINPQK DETLETEKAQ YYLPDGSTIE IGPSRFRAPE LLFRPDLIGE ESEGIHEVLV FAIQKSDMDL RRTLFSNIVL SGGSTLFKGF GDRLLSEVKK LAPKDVKIRI SAPQERLYST WIGGSILASL DTFKKMWVSK KEYEEDGARS IHRKTF
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ACTR1A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ACTR1A Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ACTR1A (Alpha Centractin (ACTR1A))
    Alternative Name
    ACTR1A (ACTR1A Products)
    Synonyms
    ARP1 Protein, CTRN1 Protein, Arp1 Protein, alpha-Arp1 Protein, arp1 Protein, centractin Protein, ctrn1 Protein, ACTR1A Protein, ARP1 actin related protein 1 homolog A Protein, ARP1 actin-related protein 1A, centractin alpha Protein, ARP1 actin-related protein 1 homolog A, centractin alpha Protein, ARP1 actin related protein 1 homolog A L homeolog Protein, alpha-centractin Protein, actin-2 Protein, ACTR1A Protein, Actr1a Protein, actr1a.L Protein, actr1a Protein, PTRG_02540 Protein, PAAG_06972 Protein, MCYG_01044 Protein, VDBG_00685 Protein, MGYG_00946 Protein, DICPUDRAFT_88548 Protein, PGTG_04034 Protein, Tsp_06409 Protein
    Background
    Component of a multi-subunit complex involved in microtubule based vesicle motility. It is associated with the centrosome.
    Molecular Weight
    43.6 kDa Including tag.
    UniProt
    P61163
    Pathways
    M Phase
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