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A2ML1 Protein (AA 18-1454) (His tag)

Crystallography grade A2ML1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3088387
  • Target See all A2ML1 Proteins
    A2ML1 (alpha-2-Macroglobulin-Like 1 (A2ML1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 18-1454
    Origin
    Human
    Source
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This A2ML1 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    EELPNYLVTL PARLNFPSVQ KVCLDLSPGY SDVKFTVTLE TKDKTQKLLE YSGLKKRHLH CISFLVPPPA GGTEEVATIR VSGVGNNISF EEKKKVLIQR QGNGTFVQTD KPLYTPGQQV YFRIVTMDSN FVPVNDKYSM VELQDPNSNR IAQWLEVVPE QGIVDLSFQL APEAMLGTYT VAVAEGKTFG TFSVEEYVLP KFKVEVVEPK ELSTVQESFL VKICCRYTYG KPMLGAVQVS VCQKANTYWY REVEREQLPD KCRNLSGQTD KTGCFSAPVD MATFDLIGYA YSHQINIVAT VVEEGTGVEA NATQNIYISP QMGSMTFEDT SNFYHPNFPF SGKIRVRGHD DSFLKNHLVF LVIYGTNGTF NQTLVTDNNG LAPFTLETSG WNGTDVSLEG KFQMEDLVYN PEQVPRYYQN AYLHLRPFYS TTRSFLGIHR LNGPLKCGQP QEVLVDYYID PADASPDQEI SFSYYLIGKG SLVMEGQKHL NSKKKGLKAS FSLSLTFTSR LAPDPSLVIY AIFPSGGVVA DKIQFSVEMC FDNQVSLGFS PSQQLPGAEV ELQLQAAPGS LCALRAVDES VLLLRPDREL SNRSVYGMFP FWYGHYPYQV AEYDQCPVSG PWDFPQPLID PMPQGHSSQR SIIWRPSFSE GTDLFSFFRD VGLKILSNAK IKKPVDCSHR SPEYSTAMGA GGGHPEAFES STPLHQAEDS QVRQYFPETW LWDLFPIGNS GKEAVHVTVP DAITEWKAMS FCTSQSRGFG LSPTVGLTAF KPFFVDLTLP YSVVRGESFR LTATIFNYLK DCIRVQTDLA KSHEYQLESW ADSQTSSCLC ADDAKTHHWN ITAVKLGHIN FTISTKILDS NEPCGGQKGF VPQKGRSDTL IKPVLVKPEG VLVEKTHSSL LCPKGKVASE SVSLELPVDI VPDSTKAYVT VLGDIMGTAL QNLDGLVQMP SGCGEQNMVL FAPIIYVLQY LEKAGLLTEE IRSRAVGFLE IGYQKELMYK HSNGSYSAFG ERDGNGNTWL TAFVTKCFGQ AQKFIFIDPK NIQDALKWMA GNQLPSGCYA NVGNLLHTAM KGGVDDEVSL TAYVTAALLE MGKDVDDPMV SQGLRCLKNS ATSTTNLYTQ ALLAYIFSLA GEMDIRNILL KQLDQQAIIS GESIYWSQKP TPSSNASPWS EPAAVDVELT AYALLAQLTK PSLTQKEIAK ATSIVAWLAK QHNAYGGFSS TQDTVVALQA LAKYATTAYM PSEEINLVVK STENFQRTFN IQSVNRLVFQ QDTLPNVPGM YTLEASGQGC VYVQTVLRYN ILPPTNMKTF SLSVEIGKAR CEQPTSPRSL TLTIHTSYVG SRSSSNMAIV EVKMLSGFSP MEGTNQLLLQ QPLVKKVEFG TDTLNIYLDE LIKNTQTYTF TISQSVLVTN LKPATIKVYD YYLPDEQATI QYSDPCE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human A2ML1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product A2ML1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    A2ML1 (alpha-2-Macroglobulin-Like 1 (A2ML1))
    Alternative Name
    A2ML1 (A2ML1 Products)
    Synonyms
    CPAMD9 Protein, alpha-2-macroglobulin like 1 Protein, A2ML1 Protein
    Background
    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity). Displays inhibitory activity against chymotrypsin, papain, thermolysin, subtilisin A and, to a lesser extent, elastase but not trypsin. May play an important role during desquamation by inhibiting extracellular proteases. {ECO:0000250|UniProtKB:P01023, ECO:0000269|PubMed:16298998}.
    Molecular Weight
    160.3 kDa Including tag.
    UniProt
    A8K2U0
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