Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

PRMT3 Protein (AA 2-531) (His tag)

Crystallography grade PRMT3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Catalog No. ABIN3088812
  • Target See all PRMT3 Proteins
    PRMT3 (Protein Arginine Methyltransferase 3 (PRMT3))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-531
    Origin
    • 3
    • 1
    • 1
    Human
    Source
    • 1
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This PRMT3 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
    Sequence
    CSLASGATGG RGAVENEEDL PELSDSGDEA AWEDEDDADL PHGKQQTPCL FCNRLFTSAE ETFSHCKSEH QFNIDSMVHK HGLEFYGYIK LINFIRLKNP TVEYMNSIYN PVPWEKEEYL KPVLEDDLLL QFDVEDLYEP VSVPFSYPNG LSENTSVVEK LKHMEARALS AEAALARARE DLQKMKQFAQ DFVMHTDVRT CSSSTSVIAD LQEDEDGVYF SSYGHYGIHE EMLKDKIRTE SYRDFIYQNP HIFKDKVVLD VGCGTGILSM FAAKAGAKKV LGVDQSEILY QAMDIIRLNK LEDTITLIKG KIEEVHLPVE KVDVIISEWM GYFLLFESML DSVLYAKNKY LAKGGSVYPD ICTISLVAVS DVNKHADRIA FWDDVYGFKM SCMKKAVIPE AVVEVLDPKT LISEPCGIKH IDCHTTSISD LEFSSDFTLK ITRTSMCTAI AGYFDIYFEK NCHNRVVFST GPQSTKTHWK QTVFLLEKPF SVKAGEALKG KVTVHKSKKD PRSLTVTLTL NNSTQTYGLQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PRMT3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    PRMT3 (Protein Arginine Methyltransferase 3 (PRMT3))
    Alternative Name
    PRMT3 (PRMT3 Products)
    Synonyms
    HRMT1L3 Protein, 2010005E20Rik Protein, 2410018A17Rik Protein, AL033309 Protein, Hrmt1l3 Protein, PRMT3 Protein, hrmt1l3 Protein, zgc:112498 Protein, ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 3 Protein, ATPRMT3 Protein, protein arginine methyltransferase 3 Protein, protein arginine methyltransferase 3 Protein, protein arginine N-methyltransferase 3 Protein, protein arginine methyltransferase 3 S homeolog Protein, PRMT3 Protein, Prmt3 Protein, prmt3 Protein, prmt3.S Protein
    Background
    Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.
    Molecular Weight
    60.7 kDa Including tag.
    UniProt
    O60678
    Pathways
    Protein targeting to Nucleus
You are here:
Support