APOBR Protein (AA 1-1088) (His tag)

Catalog No. ABIN3088997

Product Details

Target: APOBR (Apolipoprotein B Receptor (APOBR))

Protein Type: Recombinant

Protein Characteristics: AA 1-1088

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This APOBR protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: MDFLRLYLPG LHQALRGALD SLGTFVSYLL GDAVPTVERE AQAAEELGVV AVGKTGKIVE EEAQEDLEGL RGSQNEGAGR LRGPGDDRRH EVGSSAVEQT WGWGDGSSHG SQAERQDSGA GETAKAARCQ EPSAHLEARK KSKAGSGACQ DRSGQAQERQ ESHEQEVNRE ERLRSWEQEE EEEEVRAREP GMARGAESEW TWHGETEGKA GAVGPKAAGD NREMEQGVRE ADAGETEEPG AEGAGKGEEV VVVEKACEST RAWGTWGPGA EPEDWGILGR EEARTTPGRE EARAILDGEE ARTISGGEEA ETASGGEEAE TASGGEEAGT ASGGEEAGIA SGGEAGTASG GEEAGTASGG DEAWTTSGKE EADLLGVRQT EYGAVPGERL LEATGKVWVL EEEGDEEREA EVSPFPKQPQ VLGTERTEEA AESQTAGREA VGGQEAGESF EGQVDLRGKE AEMRQDLGIR ADRARMEELV QAEEAQEERG SSRDPVAELP SDGEAEGTAD LEATPEARPE EELTGEESEA AQTSCGLLGV EWGGLTHSVT KGQGPELMGG AQTPTKQPEE REAGEVELMG VLALSKEEQE RSLEAGPRHA GSVKPEASEA FPGAWENRTR KDMERGNTQE DAADGEQREE EETAGGQTLA AEAEGDRESE LSEVPEAGGE GLTTQDAGCG TEEGEASVSE NQELDGSTGA DAGPCPSLGE AYARETEDEE AEADRTSRRG WRLQAVAVGL PDREDAQTGS VAAGIMGGDV VPHISAAGAG EALEGVLGQG WDSKEKEEAA AGEHAGGQEF GLEGSAEEEV TGRGSQVEAF ESREGGPWGG RVEAEESAGA EDSCGLDPAG SQTARAEGMG AMVEAGGLLE KWTLLEEEAV GWQEREQRED SEGRCGDYHP EGEAPRLLDA EGLMVTGGRR AEAKETEPES LEHVRGQEEQ PTHQAPAEAA PESVGEAETA EAMGSARGGA ANSWSEAPLP GSLLDVSVPR SRVHLSRSSS QRRSRPSFRR TPAWEQQEEP PAPNPPEEEL SAPEQRPLQL EEPLEPSPLR HDGTPVPARR RPLGHGFGLA HPGMMQELQA RLGRPKPQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human APOBR Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: APOBR (Apolipoprotein B Receptor (APOBR))

Alternative Name: APOBR (APOBR Products)

Synonyms: APOB48R Protein, Apob-48r Protein, Apob48r Protein, RGD1565169 Protein, APOB100R Protein, apolipoprotein B receptor Protein, apolipoprotein B48 receptor Protein, APOBR Protein, LOC745786 Protein, Apobr Protein

Background: Macrophage receptor that binds to the apolipoprotein B48 (APOB) of dietary triglyceride (TG)-rich lipoproteins (TRL) or to a like domain of APOB in hypertriglyceridemic very low density lipoprotein (HTG-VLDL). Binds and internalizes TRL when out of the context of the macrophage. May provide essential lipids to reticuloendothelial cells. Could also be involved in foam cell formation with elevated TRL and remnant lipoprotein (RLP). Mediates the rapid high-affinity uptake of chylomicrons (CM), HTG-VLDL, and trypsinized (tryp) VLDL devoid of APOE in vitro in macrophages. {ECO:0000269|PubMed:10852956, ECO:0000269|PubMed:15591219, ECO:0000269|PubMed:9633939}.

Molecular Weight: 115.8 kDa Including tag.

UniProt: Q0VD83