ATP1A2 Protein (AA 6-1020) (rho-1D4 tag)

Catalog No. ABIN3089524

Product Details

Target: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Protein Type: Recombinant

Protein Characteristics: AA 6-1020

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This ATP1A2 protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: GREYSPAATT AENGGGKKKQ KEKELDELKK EVAMDDHKLS LDELGRKYQV DLSKGLTNQR AQDVLARDGP NALTPPPTTP EWVKFCRQLF GGFSILLWIG AILCFLAYGI QAAMEDEPSN DNLYLGVVLA AVVIVTGCFS YYQEAKSSKI MDSFKNMVPQ QALVIREGEK MQINAEEVVV GDLVEVKGGD RVPADLRIIS SHGCKVDNSS LTGESEPQTR SPEFTHENPL ETRNICFFST NCVEGTARGI VIATGDRTVM GRIATLASGL EVGRTPIAME IEHFIQLITG VAVFLGVSFF VLSLILGYSW LEAVIFLIGI IVANVPEGLL ATVTVCLTLT AKRMARKNCL VKNLEAVETL GSTSTICSDK TGTLTQNRMT VAHMWFDNQI HEADTTEDQS GATFDKRSPT WTALSRIAGL CNRAVFKAGQ ENISVSKRDT AGDASESALL KCIELSCGSV RKMRDRNPKV AEIPFNSTNK YQLSIHERED SPQSHVLVMK GAPERILDRC STILVQGKEI PLDKEMQDAF QNAYMELGGL GERVLGFCQL NLPSGKFPRG FKFDTDELNF PTEKLCFVGL MSMIDPPRAA VPDAVGKCRS AGIKVIMVTG DHPITAKAIA KGVGIISEGN ETVEDIAARL NIPMSQVNPR EAKACVVHGS DLKDMTSEQL DEILKNHTEI VFARTSPQQK LIIVEGCQRQ GAIVAVTGDG VNDSPALKKA DIGIAMGISG SDVSKQAADM ILLDDNFASI VTGVEEGRLI FDNLKKSIAY TLTSNIPEIT PFLLFIIANI PLPLGTVTIL CIDLGTDMVP AISLAYEAAE SDIMKRQPRN SQTDKLVNER LISMAYGQIG MIQALGGFFT YFVILAENGF LPSRLLGIRL DWDDRTMNDL EDSYGQEWTY EQRKVVEFTC HTAFFASIVV VQWADLIICK TRRNSVFQQG MKNKILIFGL LEETALAAFL SYCPGMGVAL RMYPLKVTWW FCAFPYSLLI FIYDEVRKLI LRRYPGGWVE KETYY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ATP1A2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))

Alternative Name: ATP1A2 (ATP1A2 Products)

Synonyms: C11orf29 Protein, TIF1D Protein, TIF1DELTA Protein, AW060654 Protein, Atpa-3 Protein, mKIAA0778 Protein, RATATPA2 Protein, FHM2 Protein, MHP2 Protein, atp1a2 Protein, atp[a]2 Protein, atpa2 Protein, cb702 Protein, fa10d04 Protein, fa10d05 Protein, wu:fa10d05 Protein, ATP2A Protein, atp1a2.L Protein, fhm2 Protein, mhp2 Protein, ATP1A2 Protein, ATP1A4 Protein, tripartite motif containing 66 Protein, ATPase, Na+/K+ transporting, alpha 2 polypeptide Protein, ATPase Na+/K+ transporting subunit alpha 2 Protein, ATPase, Na+/K+ transporting, alpha 2a polypeptide Protein, sodium/potassium-transporting ATPase subunit alpha-2 Protein, ATPase, Na+/K+ transporting, alpha 2 polypeptide S homeolog Protein, TRIM66 Protein, Atp1a2 Protein, ATP1A2 Protein, atp1a2a Protein, atp1a2.S Protein, LOC488636 Protein

Background: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients.

Molecular Weight: 113.0 kDa Including tag.

UniProt: P50993

Pathways: Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process