CPA6 Protein (AA 130-437) (His tag)
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- Target See all CPA6 Proteins
- CPA6 (Carboxypeptidase A6 (CPA6))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 130-437
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This CPA6 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
- Sequence
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SLSGYNYEVY HSLEEIQNWM HHLNKTHSGL IHMFSIGRSY EGRSLFILKL GRRSRLKRAV WIDCGIHARE WIGPAFCQWF VKEALLTYKS DPAMRKMLNH LYFYIMPVFN VDGYHFSWTN DRFWRKTRSR NSRFRCRGVD ANRNWKVKWC DEGASMHPCD DTYCGPFPES EPEVKAVANF LRKHRKHIRA YLSFHAYAQM LLYPYSYKYA TIPNFRCVES AAYKAVNALQ SVYGVRYRYG PASTTLYVSS GSSMDWAYKN GIPYAFAFEL RDTGYFGFLL PEMLIKPTCT ETMLAVKNIT MHLLKKCP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CPA6 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product CPA6 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- CPA6 (Carboxypeptidase A6 (CPA6))
- Alternative Name
- CPA6 (CPA6 Products)
- Synonyms
- MGC53632 Protein, CPAH Protein, ETL5 Protein, FEB11 Protein, 9030616D13Rik Protein, carboxypeptidase A6 L homeolog Protein, carboxypeptidase A6 Protein, carboxypeptidase Protein, cpa6.L Protein, CPA6 Protein, SJAG_00451 Protein, cpa6 Protein, LOC100543921 Protein, Cpa6 Protein
- Background
- May be involved in the proteolytic inactivation of enkephalins and neurotensin in some brain areas. May convert inactive angiotensin I into the biologically active angiotensin II. {ECO:0000269|PubMed:18178555}.
- Molecular Weight
- 37.2 kDa Including tag.
- UniProt
- Q8N4T0
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