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CCDC65 Protein (AA 1-484) (Strep Tag)

Crystallography grade CCDC65 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Catalog No. ABIN3091199
  • Target See all CCDC65 Proteins
    CCDC65 (Coiled-Coil Domain Containing 65 (CCDC65))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-484
    Origin
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 3
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This CCDC65 protein is labelled with Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequence
    MPKKEKMAKT PLSDEKQLLL FQQKLLAEEE MAKKKERLLS QFLKDKLAKE EHNSALNLNK INTQWRTVLR EVKTRELHKD IEILSQTFER VVDCKDNVIK SLAKDLSEAE EQYAHALRSH LHNVDQLLAL QRHRLSLLEE SYNMELEALT KEFETERKTI IDQHEKEIHY LQDIFMAMEQ NYIDSEYESK LEFQSMWNDL KNMNLEEKHF LRLHLENRVE DLWRKFQDVL KNYTDATEDR KAAFETLQVK DEKSSKEIEV QMKKIQKLQD AITISKGKIM IHSRESEDEN RYIRNDKELV LVQLRKLKAQ RTQARAASQK NLVRLTLESN ATLKALRKIV DKGEKILKLA EICRKFETEE EKVLPFYSSV LTPKEQEGIQ KNNLEELTEE LTKVMVDYIG MENFWKRYNK VKLEQLSLQH RRAQLLDING KLREMLKQYL DGISVSDEVL SQLNPLFIVN YQSNLLQPLS IRIAHPGDKQ HPTT
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Grade
    Crystallography grade
    Top Product
    Discover our top product CCDC65 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CCDC65 (Coiled-Coil Domain Containing 65 (CCDC65))
    Alternative Name
    CCDC65 (CCDC65 Products)
    Synonyms
    NYD-SP28 Protein, zgc:92302 Protein, si:dz202l16.3 Protein, si:busm1-202l16.3 Protein, 4933417K04Rik Protein, AI428139 Protein, AU020178 Protein, coiled-coil domain containing 65 Protein, ccdc65 Protein, CCDC65 Protein, Ccdc65 Protein
    Background
    Dynein regulatory complex subunit 2 (Coiled-coil domain-containing protein 65) (Testis development protein NYD-SP28),FUNCTION: Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes (By similarity). Plays a critical role in the assembly of N-DRC and also stabilizes the assembly of multiple inner dynein arms and radial spokes. Coassembles with DRC1 to form a central scaffold needed for assembly of the N-DRC and its attachment to the outer doublet microtubules (PubMed:24094744). {ECO:0000250|UniProtKB:A8JB22, ECO:0000269|PubMed:24094744}.
    Molecular Weight
    57.3 kDa
    UniProt
    Q8IXS2
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