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CNTNAP4 Protein (AA 26-1241) (His tag)

Crystallography grade CNTNAP4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3091799
  • Target See all CNTNAP4 Proteins
    CNTNAP4 (Contactin Associated Protein-Like 4 (CNTNAP4))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 26-1241
    Origin
    • 4
    • 3
    Human
    Source
    • 3
    • 3
    • 1
    Insect Cells
    Purification tag / Conjugate
    This CNTNAP4 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    GNSYDCDDPL VSALPQASFS SSSELSSSHG PGFARLNRRD GAGGWSPLVS NKYQWLQIDL GERMEVTAVA TQGGYGSSNW VTSYLLMFSD SGWNWKQYRQ EDSIWGFSGN ANADSVVYYR LQPSIKARFL RFIPLEWNPK GRIGMRIEVF GCAYRSEVVD LDGKSSLLYR FDQKSLSPIK DIISLKFKTM QSDGILLHRE GPNGDHITLQ LRRARLFLLI NSGEAKLPST STLVNLTLGS LLDDQHWHSV LIQRLGKQVN FTVDEHRHHF HARGEFNLMN LDYEISFGGI PAPGKSVSFP HRNFHGCLEN LYYNGVDIID LAKQQKPQII AMGNVSFSCS QPQSMPVTFL SSRSYLALPD FSGEEEVSAT FQFRTWNKAG LLLFSELQLI SGGILLFLSD GKLKSNLYQP GKLPSDITAG VELNDGQWHS VSLSAKKNHL SVAVDGQMAS AAPLLGPEQI YSGGTYYFGG CPDKSFGSKC KSPLGGFQGC MRLISISGKV VDLISVQQGS LGNFSDLQID SCGISDRCLP NYCEHGGECS QSWSTFHCNC TNTGYRGATC HNSIYEQSCE AYKHRGNTSG FYYIDSDGSG PLEPFLLYCN MTETAWTIIQ HNGSDLTRVR NTNPENPYAG FFEYVASMEQ LQATINRAEH CEQEFTYYCK KSRLVNKQDG TPLSWWVGRT NETQTYWGGS SPDLQKCTCG LEGNCIDSQY YCNCDADRNE WTNDTGLLAY KEHLPVTKIV ITDTGRLHSE AAYKLGPLLC QGDRSFWNSA SFDTEASYLH FPTFHGELSA DVSFFFKTTA SSGVFLENLG IADFIRIELR SPTVVTFSFD VGNGPFEISV QSPTHFNDNQ WHHVRVERNM KEASLQVDQL TPKTQPAPAD GHVLLQLNSQ LFVGGTATRQ RGFLGCIRSL QLNGMTLDLE ERAQVTPEVQ PGCRGHCSSY GKLCRNGGKC RERPIGFFCD CTFSAYTGPF CSNEISAYFG SGSSVIYNFQ ENYLLSKNSS SHAASFHGDM KLSREMIKFS FRTTRTPSLL LFVSSFYKEY LSVIIAKNGS LQIRYKLNKY QEPDVVNFDF KNMADGQLHH IMINREEGVV FIEIDDNRRR QVHLSSGTEF SAVKSLVLGR ILEHSDVDQD TALAGAQGFT GCLSAVQLSH VAPLKAALHP SHPDPVTVTG HVTESSCMAQ PGTDATSRER THSFADHSGT IDDREPLANA IKSDSA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CNTNAP4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product CNTNAP4 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CNTNAP4 (Contactin Associated Protein-Like 4 (CNTNAP4))
    Alternative Name
    CNTNAP4 (CNTNAP4 Products)
    Synonyms
    CNTNAP4 Protein, CNTNAP5 Protein, cntnap4 Protein, CASPR4 Protein, Caspr4 Protein, E130114F09Rik Protein, contactin associated protein like 4 Protein, contactin-associated protein-like 3 Protein, contactin-associated protein-like 4 Protein, contactin associated protein like 3 Protein, contactin associated protein-like 4 Protein, CNTNAP4 Protein, LOC100064076 Protein, LOC100472293 Protein, cntnap3 Protein, cntnap4 Protein, Cntnap4 Protein
    Background
    Presynaptic protein involved in both dopaminergic synaptic transmission and GABAergic system, thereby participating in the structural maturation of inhibitory interneuron synapses. Involved in the dopaminergic synaptic transmission by attenuating dopamine release through a presynaptic mechanism. Also participates in the GABAergic system (By similarity). {ECO:0000250}.
    Molecular Weight
    135.9 kDa Including tag.
    UniProt
    Q9C0A0
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